The Pharmacodynamics Study Of Matrine Gutta To Cure Bacterial Keratitis And Bacterial Conjunctivitis

Matrine is the main natural base of Sophora flavescens Ait.It has pharmaco-activity and admove future in central nervous system, cardiovascular system,to reject virus,antiinflammatory,immunize and to reject tumor and so on.The molecular formula of matrine is C₁₅H₂₄N₂O,the molecular mass is 248.36,it is isom of lupanine,tetracyclic quinolizidine,the skeleton molecule is two thick zoarium of quinolizidine,matrine is white crystal,nofoul,bitter in taste,there is hygroscopicity and to change amber long in air.1.the pharmaco-study of matrine1)central nervous system:to reject convulsion,analgesia,hypnotize2)cardiovascular system:positive inotropic effect,to reject upset heart rate,to reject atherosis,to reject vascular remodeling of high blood pressure3)systema digestorium:to reject trauma of stomach's mucosa choleretic effect,to check diarrhea,to reject Injury of liver4)systema genitale:antifertility by inhibit and to kill sperm5)anti-inflammatory effect:to reject a lot of Acute inflammation6)ob-dermatofibrosis:obviate and therapeutical7)prevention and cure brain edema:preventive and therapeutic effect to cure brain edema of rat8)immunosuppressive action:to cure immune disease9)antibacterial activity:to reject many bacteria10)to reject virus:to reject CVB₃11)to reject tumor:inhibit cell proliferation of tumor2.vitro antibiosis experiment of matrine 1)minimal inhibitory concentration(MIC)To determine MIC of Staphylococcus aureus,Pseudomonas aeruginosa, Bacterium coli by tube dilution method.Prepare matrine to 40000,20000,10000,5000,2500,1250,625,312.5,156.25,78.125 ug·ml^-1with LB bouillon by tube dilution method.Set up 21 group,1-10 group:40000μg.ml^-1-78.6251μg.ml^-110 density physic liquor,at the same time,add 1 ml bacterium liquid,5 test tube in one group.11-20 group:only put 40000μg.ml^-1-78.6251μg.ml^-110 density physic liquor,5 test tube in one group.21 group:only put bacterium liquid,5 test tube in one group.37℃shaking culture for 16～18 h,the minimum density which has no Bacterium(clear)is minimal inhibitory concentration(MIC).2)minimal inhibitory concentration MBCTo determine MBC of Staphylococcus aureus,Pseudomonas aeruginosa,Bacterium coli by tube dilution method.Prepare matrine to 40000,20000,10000,5000,2500,1250,625,312.5,156.25,78.125ug·ml^-1with LB bouillon by tube dilution method.To put different tiny test tube,at the same time,add 1 ml bacterium liquid,5 test tube in one group.Having an inoculum liquid, 37℃shaking culture for 16～18 h.To observe minimal inhibitory concentration(MIC)of every culture.To take every suction 0.1ml and to move common agar culture.Spread on bacterium liquid with glass rod,to put 37℃culture after one night.In the plate cultivation med,To count few 5 piece colony who should have minimum bactericidal concentration(MBC).3)experimental resultTo determine MIC of Staphylococcus aureus,Pseudomonas aeruginosa,Bacterium coli by tube dilution method.The result is 10000μg·ml^-1.To determine MBC of Staphylococcus aureus,Pseudomonas aeruginosa,Bacterium coli by tube dilution method.The result is 20000μg·ml^-1.3.Vivo experiment of matrine1)The model building of rabbit's keratitis and taraxisTo breed the experimental animal for one week.Checking two eyes,no abnormality seen.To make corneal trephine method(except normal control). Weigh before experiment,drop into eyes 2～3 with 0.75%bupivacaine hydrochloride and light massage,to result in orbit hurt by 5mm and 3mm corneal trephine.Pull eyelid away calathiform,imbibe Staphylococcus aureus(33 x 10⁷·ml^-1), Pseudomonas aeruginosa(23 x 10⁷·ml^-1),Bacterium coli(23 x 10⁷·ml^-1)by 5 ml syringe,drop 0.1 ml,close eyelid and massage for a minute,to catch simple eye.2)divide into groups and dosagedivide experimental animal into 16 groups,6 in a group.Dosage arabbit one vices each 2 h,6 vices each day,0.1ml per vices for 10 days.give physiological saline to the rabbit of bacterial infection control group every day.3)the experimental result of bacterial keratitisEach group of ceratitis to catch by Staphylococcus aures,the obviously cure efficiency ofmatrine:66.7%in low group;66.7%in middle group;88.3%in high group.Effective power:100%,the obviously cure efficiency of masc drug treatment group is 66.7%and effective power is 100%.Each group of ceratitis to catch by Pseudomonas aeruginosa,the obviously cure efficiency of matrine is 66.7%in group,the obviously cure efficiency ofmasc drug treatment group is 50%and effective power is 100%.Each group of ceratitis to catch by Bacterium coil,the obviously cure efficiency of matrine is 88.3%in group,effective power is 100%.the obviously cure efficiency of masc drug treatment group is 66.7%and effective power is 100%.4)the experimental result of bacterial conjunctivitisEach group of conjunctivitis to catch by Staphylococcus aureus the obviously cure efficiency of matrine:83.3%in low group;100%in middle group;100%in high group.Effective power:100%,the obviously cure efficiency of masc drug treatment group is 66.7%and effective power is 100%.Each group of conjunctivitis to catch by Bacterium coli,the obviously cure efficiency of matrine is 100%in group,effective power is 100%.the obviously cure efficiency of masc drug treatment group is 83.3%and effective power is 100%...