| Aim: T-cells Play an important role in transplant rejection, graft-versus-host reaction, autoimmune and tumor immunity. Delayed-type hypersensitivity (DTH) in mice is a specific T cell-mediated immune response.To confirm whether glucosides of Chaenomeles speciosa (GCS) and catechins (Cat) can regulate cell immunity of mice, and to explore whether Cat is one of the main active ingredient of GCS, the study used DTH induced by 2, 4-dinitrochlorobenzene (DNCB) and cyclophosphamide (Cy) potentiated or suppressed DTH in mice as a model of immune dysfunction to observe the impact of GCS and Cat on the above-mentioned model from the level of the whole, the thymocytes proliferation and cytokines produced by thymocytes.Methods: DTH of mice was induced by DNCB. The potentiated and suppressed DTH of mice was developed by Cy at a dose of 250 mg·kg-1 3 days prior to the sensitization by DNCB and 125 mg·kg-1 simultaneously with the sensitization of the mice by DNCB respectively. GCS (60, 120, 240 mg·kg-1) and Cat (30, 60, 120 mg·kg-1) were given intragastrically (ig) 3 days prior to the sensitization once daily for 7 consecutive days. Ear swelling degree, thymus index and spleen index was used as evaluating indication. Thymus lymphocyte proliferation was induced by concanavalin A (ConA) to examine the influence of different doses or concentrations of GCS and Cat on the lymphocyte proliferation and secretion of interleukin-2 and interleukin-4 in vivo or vitro. ConA-induced thymocytes proliferation was measured by 3-(4,5-dimethylthiazol-2- -thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) assay. IL-2 activity was measured by testing its ability to support ConA-induced mice thymocytes proliferation by the MTT assay. IL-4 were measured by enzyme-linked immunosorbent assay(ELISA).Results:1. the effects on cell immunity of DTH mice by GCS and Cat. GCS and Cat could significantly inhibite DTH response in Normal mice. GCS (60, 120, 240 mg·kg-1) and Cat (120 mg·kg-1) could significantly decrease the ear swelling; GCS (120, 240 mg·kg-1) and Cat (120 mg·kg-1) could significantly decrease the thymus index and the spleen index; GCS (60, 120, 240 mg·kg-1) and Cat (60, 120 mg·kg-1) could decrease thymocytes proliferation and IL-2 levels to close to the Normal levels; GCS (120, 240 mg·kg-1) and Cat (120 mg·kg-1) could significantly increase IL-4 levels in cultures of thymocytes from DTH mice.In vitro, GCS (12.5～200μg·ml-1) and Cat (25～200μg·ml-1) could significantly decrease thymocytes proliferation and IL-2 levels. GCS (25～200μg·ml-1) and Cat (50～200μg·ml-1) could significantly increase IL-4 levels in cultures of thymocytes from DTH mice with a concentration-dependent dose-effect relationship.2. the effects on cell immunity of Cy- potentiated DTH mice by GCS and Cat. GCS and Cat could significantly inhibite DTH response in Cy-potentiated DTH mice. GCS (60, 120, 240 mg·kg-1) and Cat (120 mg·kg-1) could significantly decrease the ear swelling of Cy-potentiated DTH mice; GCS (120, 240 mg·kg-1) and Cat (120 mg·kg-1) could significantly decrease the thymus index of Cy-potentiated DTH mice; GCS (60, 120, 240 mg·kg-1) and Cat (60, 120 mg·kg-1) could significantly decrease the spleen index of Cy-potentiated DTH mice; GCS (120, 240 mg·kg-1) and Cat(60, 120 mg·kg-1) could significantly decrease thymocytes proliferation; GCS (60, 120, 240 mg·kg-1) and Cat (30, 60, 120 mg·kg-1) could significantly decrease the IL-2 levels in the culture supernatants of thymocytes; GCS (120, 240 mg·kg-1) and Cat (120 mg·kg-1) could significantly increase IL-4 levels in cultures of thymocytes from the Cy- potentiated DTH mice.In vitro, GCS (12.5～200μg·ml-1) and Cat (25～200μg·ml-1) could significantly decrease thymocytes proliferation and IL-2 production of thymocytes; GCS (25～200μg·ml-1) and Cat (50～200μg·ml-1) could increase IL-4 levels in cultures of thymocytes from the Cy- potentiated DTH mice.3. the effects on cell immunity of Cy-suppressed DTH mice by GCS and Cat.GCS and Cat could enhance DTH response in Cy-suppressed DTH mice. GCS (120, 240 mg·kg-1) and Cat(60,120 mg·kg-1) could significantly increase the ear swelling of Cy-suppressed DTH mice; GCS (60, 120, 240 mg·kg-1) and Cat (60, 120 mg·kg-1) could significantly increase thymus index, spleen index of the Cy- suppressed DTH mice; GCS (60, 120, 240 mg·kg-1) and Cat (60, 120 mg·kg-1) could significantly increase thymocytes proliferation, IL-2 and IL-4 levels in cultures of thymocytes from the Cy-suppressed DTH mice.In vitro, GCS (12.5～200μg·ml-1) and Cat (25～200μg·ml-1) could significantly increase thymocytes proliferation and IL-4 production of thymocytes; GCS (12.5～200μg·ml-1) and Cat (12.5～200μg·ml-1) could significantly increase IL-2 production of thymocytes.Conclusion: GCS and Cat might have dual immunomodulatory effects in a function dependent manner in mice with immune dysfunction by regulating functions of immune organ of mice, thymocytes proliferation and the level of cytokine production by thymocytes, and balancing Th1 (IL-2) and Th2 (IL-4), Th and Ts. GCS are active compound in regulating the cell immunity of mice, Cat might regulate cell immunity of mice as one of the active ingredients of GCS.
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