Study Of Biocompatibility Of Magnesium Oxide Coating AZ31B Magnesium Alloy

ObjectiveTo investigate the biological compatibility and cytotoxicity of magnesium and it's alloy, in order to solve the key problem of poor corrosive resistance in vivo.MethodThis study prepareing magnesium alloy(AZ31B) with MgO coating by the technology of powder metallurgy and constant current anodic oxidation to definite the craft of modifing the characteristic of the magnesium alloy surface. Make the craft more easily controlled and the properties of the material more stable. Scanning the surface and the cross-section to observe the effect of Sodium Chloride on the surface and it's cross-section microstructure. To study the biocompatibility of magnesium oxide coating AZ31B magnesium alloy, we have done cytotoxicity tests, micronucleus testsand haemolysis tests in vivo on mice.ResultsThrough the surface analysis and comparison with controls, we found that oxide film can significantly reduce AZ31B particles accumulation and the formation of irregular nodules, the outer limits of the depositive grains was clear, distributeing in a uniform way. In acute and subacute tests, the mice in each group did not represent adverse reaction. There was no significant difference in weight and length between each group. In the pathological inspection of the mice liver, we had found that hepatocytes arranged normally, hepatic lobules arranged clearly and regularly, cytoplasm seemed even. It is demonstrated that the oxide film could effectively inhibit the mutagenesis in micronucleus tests. In haemolysis tests, severe hemolytic crisis(69%) was observed in the untreated AZ31B, while the suitable blood compatibility(2.5%) was shown in samples with oxide film coating on the surface. Conclusion1. AZ31Bwith oxide coating possess suitable anti-corrosion resistance. The film can control the degradation speed of magnesium and its alloy.2. AZ31B with oxide coating on it's surface have apparently cut down the haemolysis rate, and have good biocompatibility and correspond with the requires of haemolysis of biomaterial. It is demonstrated that the oxide film could effectively inhibit the effect of mutagenesis in AZ31B micronucleus tests.