| [Purpose]Established rat model of Alzheimer's disease(AD)and grafted neural stem cells(NSCs),choosing the changes of behavior test and histology of normal group,model group and transplantation group as the reference standard, discussing the value of 11C-PIB and 18F-FDG imaging in model verification and cell transplantation monitoring,hoping to providing the visualed technology method in vivo for the research of AD's diagnose and therapy.[Methods]Established the Aβhippocampus injected rat model,verified by behavior test and histology,then performed 11C-PIB PET and 18F-FDG micro PET imaging and observed whether the result of imaging was matched with behavior test and histology's.Grafted NSCs to the successful rat model,comparing the changes of behavior test and histology between model group and transplantation group,then performing the 11C-PIB and 18F-FDG imaging,observing whether the result of imaging was matched with behavior test and histology's[Result]The Morris water maze improved that the escaped latent period of model group was longer than that of normal group(P＜0.01),in histology neuron loss and Aβdeposition were found in hippocampus CAl and dentate gyrus of rat model, 11C-PIB imaging showed increased uptake in model rat hippocampus district (P＜0.05),18F-FDG imaging showed that the uptake in hippocampus inject district of model group was lower than that of normal group(P＜0.001),there was also much different uptake between bilateral hippocampus of model group(P＜0.001), these results were matched with behavior test and histology.After cell transplantation,the escaped latent period of transplantation group was shorter than that of model group(P＜0.01),which reduced 33.7%～51.5%,in histology there was no much change in Aβdeposition,NSCs differentiated and expressed ChaT,GFAP,NeuN positive cells,and expressed Brdu positive cells for six weeks, 11C-PIB imaging showed there was no much change in hippocampus district between model group and transplantation group(P＞0.05),which was matched with Aβdeposition,but not matched with results of behavior test and cell differentiation of histology.18F-FDG imaging showed there was no much chang in injected hippocampus of the two groups(P＞0.05),the result was not matched with the behavior test and cell differentiation of histology..[Conclusion]11C-PIB and 18F-FDG imaging can be the ideal of AD diagnose and monitoring the pathological change of AD model in vivo.But it can not provide a safe,dynamic,in vivo imaging tool for monitoring therapeutic effect of implant stem cell for AD at present.
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