| The mechanical stimuli from the pulsatile blood flow play a significan role in vascular remodeling. Each kind of cyclic mechanical strain includes at least three elements, magnitude, frequency, and duration. Effects of strain magnitude and exposure time on blood vessel have already been widely studied, while the mechanism of cyclic strain frequency affecting on blood vessel remains unclear. A bioreactor for blood vessel organ culture was used for studying the effects of different frequencies of pulsate flow on protein expression in organ-cultured artery in vitro. The common carotid arteries were separated freshly from Guangxi Bama mini-pigs, and cultured in the bioreactor under two different frequencies, 0.5 Hz (low frequency group) and 1.25 Hz (normal frequency group), with the mean pressure, 120 mmHg, and mean flow, 50 ml/min, for 24 hours. Total proteins were separated and analyzed by 2D gel electrophoresis and the differential proteins were identified by mass spectroscopy. The result showed that the arteries still had a good biological activity after cultured for 24 hours. 67 above-twice-differentiated- expressing proteins were found after gels analyzing, among which 49 were highly expressed in 1.25 Hz group and 18 in 0.5 Hz group. After MALDI-TOF-MS analysis, 17 proteins were identified, among which 9 had highly confident ID, Crystallin, glucose-regulated protein precursor (GRP78) and KIAA0098 were highly expressed in 0.5 Hz Group, but heat shock 27kDa protein (HSP27), triosephosphate isomerase 1, ATP synthase D chain, procollagen-proline 2-oxoglutarate 4-dioxygenase, alpha-actin, orphan nuclear receptor FOR1 isoform 2 (FOX1) were highly expressed in 1.25 Hz group. The human cultured VSMCs were subjected to mechanical cyclic strains at 0.5 Hz and 1.25 Hz frequencies respectively, and the result of Western blotting also confirmed the increasing phosphatization of HSP27 at cell level the same as tissue level, which was highly expressed in 1.25 Hz group.
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