Empirical Study On Transplantation Of Olfactory Lamina Propria Combined With Low Power Laser Irradiation For The Recovery Of Spinal Cord Injury

Objective To investigate the effects to the histology and behaviour of SD adult rat after spinal cord injury by means of transplantation of Olfactory Lamina Propria (OLP) and Low Power Laser Irradiation (LPLI) from body outside. The synergistic effect of OLP and LPLI is also investigated. Methods 32 adult SD rats randomly allocated into four groups according to factorial design: group A: OLP implantation combined with LPLI group; group B: OLP implantation group; group C: RLP (respiratory lamina propria) implantation combined with LPLI group; group D: RLP implantation group(n=8, respectively). According to BBB locomotor rating scale, we prepared the SCI model (T12) by completely transected. 4 weeks after SCI, OLP or RLP was implanted into the transected section. we observed the changes of behavior after operation, evaluated motor function of lower limbs by BBB scoring, and the changes of spine in the histology after SCI and the transference of olfactory ensheathing cells. Results There were statistical significance between OLP implantation group and RLP implantation group (F=724.765, P=0.000), BBB scores evaluation (mean is 8.281) of all OLP implantation group were higher than that (mean is 4.813) of all RLP implantation group. There were statistical significance between low power laser irradiation group and no low power laser irradiation group (F=624.059, P=0.000). BBB scores evaluation (mean is 8.156) of all LPLI group were higher than that (mean is 4.938) of all no LPLI group. Both OLP and LPLI have the synergistic action(F=119.118, P=0.000). Anti-NF staining showed a few continuous NF traversed the lesion area in group A (OLP+PLI). Anti-NGFRp75 staining showed positived OECs in the lesion area in the group and migrate toward cephalad and caudal. Retrograde tracing by fluorogold showed a few fluorogold presented in the lesion area , and cephalad in T12 transected zone. Conclusion The recovery of spinal cord injury can be greatly improved by the application of transplantation of Olfactory Lamina Propria (OLP) and Low Power Laser Irradiation(LPLI). The synergistic effect of OLP and LPLI is significant.