Study Of The Application On Pretreatment Of Biological Samples Of Propofol With Molecularly Imprinted Polymers And Membrane Separation

Objective: To prepare propofol molecular imprinting solid phase extraction columns and membrane separation device for pre-treatment propofol samples, establish a rapid, continuous and practical approach for pre-treating propofol blood samples,improve the fiber-optical fluorescence detection system developed by item team for propofol with membrane separation device integrated with molecularly imprinted solid phase extraction columns. Method: Synthesis of molecularly imprinted polymers with propofol as template by thermal and UV polymerization,preparation of propofol molecularly imprinted membrane with phase inversion and UV-triggered in-situ polymerization, study of the performance of polymers, the morphology of membrane, the reaction mechanism of polymerization and the adsorption of propofol in plasma adsorbed by Imprinted Polymers ;preparation of molecularly imprinted solid phase extraction columns with polymers, and optimization of activation, washing and elution conditions of extraction columns,compareation of the effects of propofol plasma samples pretreated by C18 columns and molecularly imprinted columns; separation of propofol in the whole blood with membrane separation approach, and selection of suitable membrane, extraction agent and pump speed;membrane separation and molecularly imprinted solid phase extraction integrated with fiber-optical fluorescence detection system developed by item team for propofol was used to analyze the concentration of propofol in blood. Results: (1) Imprinted polymers binded to propofol selectively; binding capacity of imprinted Polymers prepared by UV polymerization was higher than that prepared by thermal polymerization;the higher polymerization temperature, the more completive polymerization reaction, the corresponding poorer swell of the polymers, and the ability of identification decreased; propofol binded to functional monomer methyl acrylate with hydrogen bond and 1:1 ratio,when the distanse between UV lamps and imprinted membrane was 5 cm , prepolymer solution formated membrane quickly and completely; imprinted Polymer adsorbed part of propofol in plasma. (2) When n-hexane as activation, hexane: toluene (9:1) as eluent, methanol as eluting agent,the cumulative recovery of propofol samples treated by molecularly imprinted columns was 99.94%, the linear and accuracy of pre-treatment of series concentrations of propofol in blood with MISPE were better than that of C18 columns. (3)In the process of extracting propofol in whole blood with membrane separation approach, when the concentration of propofol in blood was 1 to 20μg.ml-1, the selection of polyethersulfone membrane for mesomerism and plasma for dialysis fluid, 5 rpm for the pump speed and 2 min for dialysis time, the average recovery rate of dialysis was 25.50%, SD was 0.54%. (4) 100 mg of polymer and 5 bar pressure of injection were choosen to analyze the concentration of propofol in whole blood with improved fiber-optical fluorescence detection system for propofol, only 4.8 minutes for analysis of every sample. Conclusion: Membrane separation device integrated with molecularly imprinted solid phase extraction columns can treat propofol samples in the whole blood online and rapidly, no blood "injury" ,this was a great improvement of fiber-optical fluorescence detection system for propofol.