Experimental Study On The Prevention Of Cataract Induced By Oxidative Damage With Tetramethylpyrazine

Objective To observe the inhibition of tetramethylpyrazine on oxidative-induced cataractous lens of rats.Materials and method: Twenty-four rats were killed and taken out of transparent lens with no injury. These lens were hatched in RPMI-1640 culture fluid for 3 hrs, then 33 transparent lens divided into 5 groups: normal control group, model group and three tetramethylpyrazine groups with different concentrations. The concentration of peroxide (H2O2) is 300μmol/L. The lens were hatched under the different conditions for 24 hrs in vitro. Three lens were examined by scanning electron microscopy. Then the transparency of the lens was observed. At the same time, the enzyme activation levels of Superoxide dismutase (SOD),Glutathione peroxidase (GSH-PX) and Catalase (CAT) were measured respectively.Result: All lens were transparent in control group, all the lens of the model group developed cataract, the lens opacity of the three protective groups with tetramethylpyrazine were not so obvious as these of the model group lens by oxidative damage. The structure of len in normal control group was normal under the scanning electron microscopy but was damaged severely in modle group, slight damages were found in the len which were protected by tetramethylpyrazine. The difference of the score of the opacity between the groups is obvious(F=9.2,P<0.05).The enzyme activation levels of SOD,GSH-PX and CAT in the model group were obviously lower than those of enzymes in normal control group(P <0.01), but the levels of them increased in tetramethylpyrazine groups compared with those in model group(P<0.01).Conclusion Tetramethylpyrazine could protect lens of rats from oxidative damage by increasing enzyme activation of SOD,GSH-PX,CAT and inhibit or delay the generation and development of peroxidate-induced cataract at the early stage.