| Objective: To investigate the possibility of survival,migration and differentiation of rat mesenchymal stem cells(rMSCs) in host myocardium , the effects of rMSCs and exogenous rMSCs which adenovirus mediate vascular endothelial growth factor 165 gene (AdVE- GF165) transfer into transplantation on the function of heart with myocardial infarction; To observe the feasibility of using exogenous adult stem cells for cell therapy.Methods: (1) Wistar Rat rMSCs were cultured in vitro, AdVEGF165 transfer it. ELISA method was applied to assay the expression and secretion of VEGF. (2) rMSCs were cultured in vitro and transferred by AdVEGF165, ELISA method was applied to assay the expression and secretion of VEGF; 30 male SD rats were separated randomly into three groups : acute myocardial infarction control group (control,n=10), myocardial infarction plus cell transplantation group (A MI+Cell,n=10) and myocardial infarction plus VEGF-transfer-cell transplantation group(AMI+VEGF-Cell,n=10).The infracted hearts were made by occlusion of left coronary artery. Male Wistar rats MSCs were isolated and purified. Then the cells were implanted into the infracted hearts of male SD rats. Heart functions were measured by ultrasound 4 weeks after implantation. The hearts were harvested 4 weeks after implantation for immunohischemistry. Results (1) ELISA show: upper liquid of transfer group has a great deal of VEGF expression and more significant than control group ( p<0.05 ) ; (2)Immunohistochemistry show: Purified rMSCs survived and homed in exogenous host hearts; (3)ultrasound show: improvement degree of EF of Group C more significant than other groups(p<0.05).Conclusion The purified rMSCs can survive in exogenous host hearts without addition of any immunosuppressant. rMSCs which were transferred AdVEGF165 take part in myocardium regeneration and have beneficial effects on heart function.
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