Effect Of VEGF165 On The Permeability Of Blood Brain Barrier After Intracerebral Hemorrhage

Objective: To study the protective effects of VEGF165 on the permeability of blood brain barrier after intracerebral hemorrhage(ICH) in rats.Methods: According to methods of Yang, Lee and Hua, a medium volume of hematoma was formed by stereotactic infusing 60μl autologous caudate artery blood. Behavioral evaluation was performed. Rats were put to death at different time points. Then VEGF protein expression was observed by immuohistochemical method. Brain water content was determined by dry-wet weight method. The BBB permeability was measured by Evans-Blue (EB) extravasion. By injecting VEGF165 into lateral ventricle, we observed the effect of VEGF165 on the permeability of BBB after cerebral hemorrhage. Results: Rats ICH model established successfully by stereotactic infusing autologous artery blood is similar to human cerebral hemorrhage patients in some extent. The expression of VEGF protein increased significantly after cerebral hemorrhage ,peaked at 7 day ,had a significant elevation compared with sham operation group(p<0.05). Behavioral evaluation of rats of group ICH treated by VEGF165 was lower clearly than that of rats of group ICH untreated(control group). Brain water content around the hematoma of VEGF165 therapy group peaked 24h after the hemorrhage, then declined gradually and declined markedly after 5days. Its change was similar to that of control group. However brain water content of VEGF165 therapy group at different time point is lower than that of control group(untreated group). EB content around the hematoma of VEGF165 therapy group peaked at 24h, and then declined. Its change pattern was similar to that of control group. However EB content around the hematoma of VEGF165 therapy group declined more than that of control group at different time point (p<0.05). The change pattern of brain water content was similar to that of the BBB permeability.Conclusion: We successfully established rat ICH model by stereotactic infusing autologous artery blood. VEGF165 had a marked protective and therapeutic effect to BBB of rat ICH.