Effects Of Selective Cyclooxygenase-2 Inhibitor Celebrex On The Growth Of Gallbladder Carcinoma GBC-SD Cell Line

Objective: To investigate the role of cyclooxygenase-2 inhibitor Celebrex on the growth of gallbladder carcinoma GBC-SD cell line.Methods: The gallbladder caicinoma cell line GBC-SD cells were cultivated in abundance. Growth suppression of 40μmol/L,80μmol/L,120μmol/L and 160μmol/L of celereb were counted by MTT method. Apoptotic index(AI) was evaluated by TUNEL staining. The apoptotic rate was counted by flow cytometry(FCM), fluorescence microscopy(FM) and transmission electron microscopy(TEM).Reluts: celebrex can inhibit the growth of GBC-SD cell line in dose-depend. The growth inhibit rate with 40μmol/L,80μmol/L,120μmol/L and 160μmol/L was 18.77%, 25.32%,46.58% and 52.19% respectively (p<0.01).Apoptotic rate of GBC-SD cell with 40μmol/L,80μmol/L,120μmol/L and 160μmol/L celebrex were (8.51±1.44)%,(12.40±0.87)%,(26.37±1.72)% and (43.21±0.39)% respectively. Apoptotic bodies and cell shrinkage ,fragmentation was observed by FM and TEM.Conclusions:1 Celebrex may play an important role in inhibiting the growth of gallbladder carcinoma GBC-SD cell line2 celebrex can inhibit the growth of GBC-SD cell line in dose-depend.