The Preparation And Applications Of AlS₄Pc NPs And Preliminary Research On Its Biological Security

This dissertation consists of four charpters.In charpter one, the varieties and preparation of nanoparticles will be introduced ,and also its application in biology and iatrology. Nanotechnology as a emerging science since 1980s ,has a vast perspective in materials, chemistry, biomedicine,and environment science. We performed some researches based on the theme of nanotechnology.AlS4Pc-core shell nanoparticles was prepared in chapter two. A fluorophore modified silica precursor is synthesized by the reaction of amino-reactive organic fluorphores, AlS4Pc with APTEOS.Then the sol-gel method is used to produce discrete fluorescent hybrid AlS4Pc -silica nanoparticles. And then we investigated the optical characters of nanoparticles, meanwhile using the nanoparticles to apply in bio-label.With the develope of fluorescent dyes technology, the bio-label technique using fluorescent dyes,has gradually replaced with isotope-label method which is dangerous because of its radiation. AlS4Pc compounded by our group has some particular features and is widely used in biological field. AlS4Pc dyes doped fluorescent nanoparticles have been prepared based on this mechanism. A fluorescence label method has also been developed by use of the AlS4Pc dyes doped fluorescent nanoparticles, which provided a new idea for the application of AlS4Pc doped fluorescent nanoparticles in biomedicine.The biological security has been studied on the AlS4Pc NPs in chapter three: Ames test which using Salmonella typhimurium was to detect the AlS4Pc NPs whether if they have mutation effects. The toxicity of AlS4Pc NPs to Hela cell was also carried on. They supported some evidence to the biocompatibility of the core-shell nanoparticles.In chapter four,TiO2 was used as a kind of photo-catalytic.TiO2 is one of the most preferable semiconductors for photo-catalytic process due to its high photosensitivity. The possibility by using anatase titanium dioxide as a photocatalyzer for the degradation of EB was investigated in this article. Factors including light sources, pH, irradiation time and the usage of catalyst that influenced the process were investigated.