Study On The Anti-inflaminatory Effect Of Effective Ingredients In PAFM

The acute and chronic pharyngitis is a throat familiar and frequently-occurring disease. Pathological Changes induced by pharyngitis can not merely cause dysfunction of thoat, but affect peripheral organs,function, even the whole body, It has a bad influence on human health. At present, drugs for treatment pharyngitis mainly in clinical application are of antibiotics, but they have lower effect and drug resistances. Consequently, it is urgent to find new drugs that have high performance and low toxicities. Traditional Chinese Medicine poses low toxicities and persistent action, becoming the hot spot of researching new drugs against pharyngitis.Physalis alkekengi L var.franchetii (Mast.) Makino has an effect of heatclearing and detoxifying, often used to treat throat gall and hoarseness. In this thesis, we got extraction from Physalis alkekengi L var.franchetii (Mast.) Makino (EPAFM) in Changbai Mountains by means of water extracting, alcohol precipitation and organic solvent extracting. Toxicities, anti- inflammation activity and reducing sputum function, immunoregulatory activity were measured in vitro and in vivo.1. Toxicity of EPAFM in vivo : the toxicity of EPAFM in vivo was measured by the method of maximum tolerated dose testing. The result showed that maximal tolerance dose of EPAFM is higher than 20g/kg.2. Antimicrobial activity of EPAFM in vitro and in vivo: Antimicrobial activity of EPAFM in vitro and in vivo are separately measured by paper piece method and bacterial culture of pharyngo swab method. The results showed that EPAFM is sensitive to S . aureus and str.Hemolyticus in vitro,and high dose group of EPAFM has a better inhibition than medium,low dose goups and watermelon frost control group.3. Anti-inflammatory activity of EPAFM: The anti-inflammatory effect of EPAFM was evaluated using the model of dimethylbenzene-induced mouse ear swelling and rat model of acute bacterial Pharyngitis. The results showed that EPAFM can significantly decrease the ear swelling degree and alleviate inflammation. In the rat model of acute bacterial Pharyngitis, The decrease of white blood cell counts in peripheral blood and inflammatory cells in the mucosa were observed. Hint: EPAFM exerted significant antiinflammatory activity4. Expectorant function of EPAFM: Expectorant experiment was conducted with methods of washing mice's respiratory tract with phenol red. The result showed that EPAFM has a nice expectorant function at the dose of 2.1g/kg.5. Immunoregulatory activities of EPAFM: Immunoregulatory activities of EPAFM were measured by the methods of organ weighter, T Lymphocyte transformation test and Level of Serum IgG. The results showed that the stimulation on immune organs proliferation and promotion of T Lymphocyte transformation function is not obvious, while its promotion of Serum IgG production.Conlusion In summary, our results indicate that the MTD of EPAFM is higher than 20g/kg. EPAFM has a nice effect of antimicrobial activity, which can effevtively inhibit the growth and propogation of many bacteria. It also has a anti-inflammation effect , restraining permeability of capillary pressure and decreasing inflammatory cells an the lesion. EPAFM plays a role immunomodulation, it can increase the content of IgG in serum and activate anti-infection immune response.