CCR7 Regulates Chemotherapy Induced By Cisplatin Through MTOR In Squamous Cancer Cells Of Head And Neck

ObjectiveTo demonstrate roTOR pathway exists in CCR7's downstream in metastatic SCCHN cell and the role of mTOR pathway in CCR7 protecting metastatic SCCHN cells against the chemotherapy induced by cisplatin.MethodsExamine the change of mTOR,p-mTOR and p-p70s6k of metastatic SCCHN cells treated by CCL19 and the inhibitors by Western Blot and RT-PCR.Examine the change of growth inhibition,cell-cycle arrest and apoptosis of metastatic SCCHN cells treated by cisplatin,CCLI9 and the inhibitors by MTT and flow cytometry.Resultsâ‘ CCL19 can not influence mTOR mRNA and total protein expression (P＞0.05).â‘¡CCL19 promotes phosphorylation of mTOR and it's substrate p70s6k, which can be decreased by the blockage of CCR7-PI3K/Akt/mTOR signal pathway (P＜0.05).â‘¢The inhibitors of CCR7-PI3K/Akt/mTOR can recover or strengthen the growth inhibition,cell-cycle arrest in G₁ phase and apoptosis induced by cisplatin in the metastatic cells pretreated by CCL19(P＜0.05).Conclusions â‘ In metastatic SCCHN cells,CCL19 can activate CCR7's downstream mTOR pathway.â‘¡The activation of CCR7 and its downstream PI3K/Akt/mTOR pathway play a critical role in metastatic SCCHN cells enduring chemotherapy.