Effects Of Selective Cyclooxygenase-2 Inhibitors On Proliferation Of Gastric Carcinoma Cell Line Bgc-823

Background and Objective:Cancer became a major public healthy problem in China and all other countries, its incidence and mortality had kept increasing since 1970s. According to the statistic data for the year 2005, the incidence and the mortality of gastric cancer was 54.5 per 100,000 person-year and 42.1 per 100,000 person-year respectively. Multiple therapies were co-operated to gastric cancer. However the number of incidence and mortality of gastric cancer was very close, suggesting that the current therapy strategies were still far from satisfaction. Many experimental and clinic studies aimed to find new effective anticancer drugs.Previous studies have shown that selective cyclooxygenase-2 inhibitors( SCIs) can inhibit cell proliferation in gastric cacinoma cell lines. However, the precise mechanism is not clear now. Many studies suggested that this effect was likely to be involved in suppression of COX-2.We designed this experiment to investigate the effect of SCIs on the proliferation and apoptosis of human Bgc-823 cells.Methods:We used the human Bgc-823 cells as target cells. The number of Bgc-823 cells was observed by means of MTT assay. Tumor opoptosis and cell cycle were studied by flow cytometry( FCM).All the detection items in this study were repeated 4 times. Statistical analysis was done using SPSS software. The data was expressed as Mean±SD. The statistical significance of the differences between control and drug- treated cells was determined by a two- tailed Student's test. P-value < 0.05 was considered as significant.Results: (1)MTT: Meloxicam and celecoxib inhibited cell proliferation in Bgc-823 cell in both concentrations-dependent manner and time-dependent manner. The effect of celecoxib was better than meloxicam's.(2)The results of FCM showed that apoptosis of Bgc-823 cells was induced after treated with various concentrations of celecoxib for 48 hours. The rate of apoptosis significantly increased with the higher concentration of celecoxib.(3)The results of FCM showed that after exposure to different concentrations of celecoxib for 48 hours, Bgc-823 cells were accumulated in G₀/G₁ phase while decreased in S phase, and these effects were in a concentration-dependent manner.Conclusions:(1)These results suggest that SCIs can inhibit cell proliferation in Bgc-823 cell in both concentrations-dependent manner and time-dependent manner. The mechanism may be involved in suppression of COX-2 expression.(2)These results suggest that SCIs influence cell cycle, induce Bgc-823 cell apoptosis in dose-dependent way.