Expression Study Of DNA Translesion Synthesis Genes In Human Primary Glioma

Background DNA translesion synthesis is a post-replication. Some researches have found that the expressions of translesion synthesis genes were up-regulated in some human common cancers, such as breast cancer, lung cancer and gastric cancer, and it means that the up-regulated expression correlated with the cancer. However, the expressions of translesion synthesis genes in human primary gliomas have never been reported.Objective This research is to detect the expressions of REV1, REV3, REV7, Polη, Polιand PolκmRNA in human primary glioma tissues and normal brain tissues, and to analyze the relationship between the expression and the pathological grade.Methods We collected 85 primary glioma tissues (20 of grade II, 20 of grade III and 45 of grade IV, according to the WHO Grading System) and 14 normal brain tissues. We choose GAPDH as the internal control, and designed primers of 6 genes and GAPDH through PRIMER 5.0. We applied SYBR Green real-time PCR to investigate the mRNA expressions of the 6 genes and the internal control gene in 85 primary glioma tissues and 14 normal brain tissues through 7900HT sequence detection system. Real-time quantitative RT-PCR data were quantified using SDS2.2 software package. Gene expression values were conducted with the formula ?Ct. We analyzed the correlation of their mRNA levels with clinical characteristics in 85 primary glioma tissues and 14 normal brain tissues to determine their roles in gliomas, including age and gender.In addition, we analyzed the correlation of the expression with the pathological grade by Spearman method.Results The expressions of 4 genes including REV3, REV7, Polηand Polιwere significantly (P<0.05) higher in glioma tissues in grade II, III and IV as compared to normal brain tissues. Among the 4 genes, the expression of REV3 was significantly (P<0.05)higher in grade IV compared to grade II and grade III glioma, the expression of Polιwas significantly (P<0.05)higher in grade IV glioma compared to grade II glioma. The expressions of REV1 and Polκwere significantly(P<0.05)higher in grade IV glioma compared to the normal brain tissue. The positive correlation existed (P<0.001) between the expression of all genes mRNA and the pathologic grade of the human glioma, except for REV7 gene. Conclusions The up-regulation of DNA translesion synthesis genes, including REV1, REV3, REV7, Polη, Polιand Polκ, may be associated with pathogenesis of glioma. The technique of SYBR Green real-time PCR is a effective multivariate technique to examine the expression of DNA translesion synthesis genes in gliomas, which can be applied to identify more tumor-specific genes.