Study On The Mutagenicity Of Traditional Vegetal Food By Fermenting

Preserved bean curd,preserved szechuan pickle,broad bean paste,lobster sauce and pickled vegetable were researched for whether possessed potential mutagenicity and what was the substance basis and influential factor of causing mutagenicity.Otherwise,the affection on mutagenicity of samples were researched by cooking with some routine methods and false gastric and intestines succus.The results indicated:1.The affection on MCN by samplesMCN of preserved bean curd had extremely significant difference(P＜0.01)with negative control.The micronuclei index of sample＞1.5 and the rang was 1.626-5.346.So preserved bean curd may have mutagenicity.MCN of preserved szechuan pickle had extremely significant difference(P＜0.01)with negate -ve control and the micronuclei index of sample＞1.5 except NO.7(5.036%0).The rang of micronuclei index was 1.033-5.716.So preserved szechuan pickle may have mutagenicity.MCN of broad bean paste had extremely significant difference(P＜0.01)with negative control and the micronuclei index of sample＞1.5.The rang of micronuclei index was 1.961-4.339.So broad bean paste may have mutagenicity.MCN of lobster sauce had extremely significant difference(P＜0.01)with negative control.The micronuclei index of sample＞1.5 and the rang was 1.961-4.339.So lobster sauce may have mutagen -icity.MCN of pickled vegetable had extremely significant difference(P＜0.01)with negative control except NO.2(7.290‰).The micronuclei index of NO.1,NO.3,NO.5,NO.7 and NO.8＞1.5.The rang of micronuclei index was 1.236-4.570.So part of them may have mutagenicity.2.The contents of histidine in samplesThe contents of histidine which was brogght in TA97,TA98 plate by samples were 8.169μg,and in TA100,TA102 plate is 8.644μg.The content≤10μg/plate had no affection on mutant colonies of TA97,TA98,TA102 by histidine and content＜15μg/plate of TA100.Results indicated that the histidine in samples had no effect on experimental results.3.The effection on mutant colonies of TA97,TA98,TA100,TA102 by samplesThe content of NO.2,NO.6 preserved szechuan pickle and NO.7,NO.8 broad bean paste for TA97 were 1,5,20,50 mg/plate.Mutant colonies of NO.6 preserved szechuan pickle were 116.33,136.0,168.33,203.67,r was 0.973 and.So NO.6 preserved szechuan pickle may have frame-shift mutagenicity.The mutant colonies hadn't reached 2 times compared with unsettled group causes by other samples.So the test of TA97 for these samples were not positive.The content of NO.2,NO.6 preserved szechuan pickle and NO.7,NO.8 broad bean paste for TA98 were 1,5,20,50 mg/plate.Mutant colonies of NO.7 broad bean paste were 92.00,93.33,104.67,130.00,r was 0.998 and mutant colonies of max content were over 2 times compared with unsettled group.Mutant colonies of NO.8 broad bean paste were 97.33,115.00,132.00,154.00,r was 0.957 and mutant colonies of max content were over 2 times compared with unsettled group.So NO.7, NO.8 broad bean paste may have frame-shift mutagenicity.The mutant colonies hadn't reached 2 times compared with unsettled group causes by other samples.So the test of TA98 for these samples were not positive.The content of NO.2,NO.6 preserved szechuan pickle and NO.7,NO.8 broad bean paste for TA100 were 12.5,25,37.5,50 mg/plate.It had no pertinence and 2 times of mutant colonies between samples and unsettled group.So the test of TA100 for these samples were not positive.The content of NO.2,NO.6 preserved szechuan pickle and NO.7,NO.8 broad bean paste for TA102 were 12.5,25,37.5,50 mg/plate.Mutant colonies of NO.2 preserved szechuan pickle were 274.33,366.67,412.33,540.33,r was 0.984 and mutant colonies of max content were over 2 times compared with unsettled group.Mutant colonies of NO.7 broad bean paste were 310.67,351.67,389.00,543.67,r was 0.959 and mutant colonies of max content were over 2 times compared with unsettled group.So NO.2 preserved szechuan pickle and NO.7 broad bean paste may have other frame-shift mutagenicity that can hardly test by these bacterium.The mutant colonies hadn't reached 2 times compared with unsettled group causes by other samples.So the test of TA102 for these samples were not positive.4.The test of mutagenic ingredients and Mutagen PrecursorsThe content ranges of nitrate,nitrite of preserved szechuan pickle and broad bean paste were 1.208-16.400 mg/kg,0.314-1.701mg/kg respectively,which were within the limitation of state hygienic standard.Secondary amine in samples were 19.10mg/kg,8.58 mg/kg,46.28 mg/kg, 69.76mg/kg that beyond relative research.There was no Sundan I in samples.The content of AFB1 were 12.66μg/kg in NO.7 broad bean paste beyond state hygienic standard and 3.00μg/kg in NO.8 broad bean paste beyond European Standard.5.The affection on mutagenicity for samples by various processMutagenicity for samples increased by fired.Mutagenicity for preserved szechuan pickle increa -sed mostly by fired with first-class rapeseed oil and broad bean paste increased mostly by fired with rapeseed oil.Mutagenicity of samples decreased by ried and boiled compared with fired samples,especially mutagenicity of preserved szechuan pickle that had no significant difference(P＞0.05)with negative control.Mutagenicity of soup which fired and boiled with preserved szechuan pickle decreased compared with unsettled control,otherwise soup of broad bean paste increased.Global aspect, mutagenicity of preserved szechuan pickle decreased and broad bean paste increased by fired and boiled.Mutagenicity for preserved szechuan pickle decreased and broad bean paste increased by microwaves.6.The affection on mutagenicity in false gastric and intestines succusMutagenicity for samples in false gastric succus increased and samples in false intestines succus decreased compared with negative control especially that they had no significant difference (P＞0.05)with negative control except NO.6 preserved szechuan pickle.Mutagenicity of NO.6 preserved szechuan pickle decreased both in false gastric succus and false intestines succus.It indicated that false gastric and intestines succus have effection with mutagenicity of samples.Mutagenicity had no significant difference(P＞0.05)between samples and samples distilled in false gastric succus,false intestines succus except NO.6 preserved szechuan pickle.Mutagenicity of NO.6 preserved szechuan pickle had extremely significant difference(P＜0.01)between direct and distilled samples in false gastric succus,false intestines succus.It proved that NO.6 preserved szechuan pickle had fat-soluble constituents.