Effects Of Low Power Laser Irradiation On Olfactory Ensheathing Cells In Vitro

Objective: To investigate the effect of 810-nm laser irradiation on the proliferation of Olfactory Ensheathing Cells in vitro. Methods: the Olfactory Ensheathing cells obtained from the adult rat olfactory mucosa using the method of the different rates of attachment were irradiated with different energy density laser light(810 nm; 10.3 mW /cm2 power output; irradiation time:30s,60s and 120s). Laser treatment was performed three times at a 24-hour interval and the cell proliferation was accessed with cell counts and methylthiazoletetrazolium (MTT) colorometric method at different time of cell growth. Results: The comparison of number of cells and average MTT values gave no statistically significant difference between all light treated groups and the control group at 3 days. The differences between all light treated groups and the control group were significant (p<0.05) except the difference in average MTT values between control and 30 s laser irradiated samples at 5 days and 8 days. Maximal effect was achieved with 60 seconds of exposure time. Conclusion: Our results showed that the low Power laser irradiation stimulates the proliferation of Olfactory Ensheathing Cells in vitro. The optimal irradiation time was found to be 60 seconds and three times at a 24-hour interval,moreover,Laser irradiation can modify cell proliferation in a dose dependent manner.