The Study Of MHC Ⅱ Expression In The Mouse Endometrial Cells

Major histocompatibility complex classⅡelements are involved in antigen presenting role of the main substances, Expressed mainly in the immune presenting cells (APC)in the immune response induced by play an important role. Studies show that MHCⅡantigen epithelial cells in the small intestine villi, airway epithelial cells have MHCⅡantigen expression, suggesting that these cells through the MHCⅡway of presenting antigen processing. Currently on the endometrium is whether the non-professional antigen-presenting cell research seldom.To explore the MHCⅡin the mouse endometrium expression and variation of test immunohistochemistry staining to detect the different physiological period of endometrial tissue in mice and cultured mouse endometrium cells and its changes at the same time measured ECM gel culture, estrogen and progesterone stimulate lymphocytes conditions and other factors to the culture medium in vitro cell mouse endometrium expression of MHCⅡ. The results show that:1. Detection of 10 different age of mouse (born after 5 d, 15 d, 30 d, 60 d, pregnancy 1 d , pregnancy 4 d, pregnancy 6 d, pregnancy 9 d, pregnancy 15 d, pregnancy 18 d) MHCⅡthe expression level. The results show that the days of the endometrial stromal cells and epithelial cells express MHCⅡwere born after 5 d, 15 d, 30 d, 60 d, with the age increase, followed by increased expression, the difference was significant ( P <0.05). 60 d, the strongest expression of MHCⅡ, but into the pregnancy decreased expression of MHCⅡ. During pregnancy, pregnancy 1 d, pregnancy 4 d, pregnancy 6 d, pregnancy 9 d these groups MHCⅡexpression of no significant difference. Pregnancy 18 d and other groups were significantly different (P <0.05), the expression of MHCⅡweakest.2. Cultured primary mouse endometrial stromal cells and epithelial cells in culture section 2～4 days positive expression of MHCⅡ, with training time, the expression of MHCⅡbeen gradually weakened to five days, tested positive for MHCⅡnegative.3. Culture in the shops on the ECM plastic mouse endometrial stromal cells and epithelial cells cultured in the first five days can still bedetected in the expression of MHCⅡ. Add estradiol and progesterone, the cells express MHCⅡthere has been no noticeable impact.4. Cultured for 5 d in the mouse endometrial stromal cells and epithelial cells in the culture me dium by adding conditions for the virus to stimulate the cell culture medium, can induce the expression of MHCⅡ.To illustrate the results of endometrial cells in the mouse immune-endocrine regulation of the role of a pilot basis.