The Establishment Of Hepatopulmonary Syndrome Model In The Rat

Objectives:To investigate the establishment of hepatopulmonary syndrome model in the rat, and the pathogenesis and development of hepatopulmonary syndrome.Metods:The model was induced in rats by chronic bile duct ligation(CBDL).Rats were studied 2wk and 5 wk after CBDL or sham surgery.The model was examined by pathology and blood gas.The expression of nitric oxide(NO),endothelin(ET)-1 and tumor necrosis factor(TNF)-αin plasma or lung tissue were detccted by colorimetric assay or enzyme linked immunosorbent assay(ELISA)using specified kit.All the detection items in this study were repeated at least 3 times.Statistical analysis was done using EXCEL software.The data was expressed as mean±SD and the statistical significance of the differences between sham and CBDL was determined by a two-tailed Student's t test.P-value＜0.05 was considered as significant.Results:HPS model rats were successfully constructed.There was a notablely increased expression of NO and ET-1 in both plasma and lung tissue in CBDL group,and TNF-αwas remarkably increased in plasma in CBDL group.Conclusions:CBDL rats can reproduce HPS model successfully.NO,ET-1 and TNF-αare probably involved in the pathogenesis of HPS.