Screeing The Differential Expression Of Drug-Resistance Related Gene To Breast Cancer By CDNA Microarray

OBJECTIVE:To study the gene differential expression of locally advanced breast cancer neoadjuvant chemotherapy and explore the relationship between gene differential expression and drug-resistance.METHODS:To obtained tissue from presurgical core needle biopsy(CNB)of primary breast tumors and surgical samples after neoadjuvant chemotherapy(paclitaxel and doxorubicin),And then used cDNA microarray representing 14,755 genes technology to identified candidate gene expression profiles of them.RESULTS:Comparison of the gene expresison proflies of before and after neoadjuvant chemotherapy,a series of genes expressed coincidentally in both gene chip were selected for drug-resistance.There are 953 genes upregulated and 433 genes downregulated.These genes relate to multiple process including apoptosis, cytoskeleton,signal conduction,cell proliferation/differentiation,regulation of cell cycle,transcription and translation of gene and metabolism of cell.The important genes upregulated were Vimentin,Cyr61,NNMT,Clusterin,AS,POLB,ERCC, The important genes downregulated were ARHI。CONCLUSIONS:These results demonstrate that drug-resistance of breast cancer is very complicated involving many gene upregulated or downregulated expression.As a new approach,cDNA microarray is feasible to genomewide exploration of gene expression patterns correlated to chemosensitivity.