The Study On Relationship Between The Expression Of Heparanase And Invasion And Metastasis In Ovarian Carcinoma

Mortality of ovarian cancer is highest in gynecological malignancies.Despite the cytoreductive surgery and platinum-based chemotherapy has played a positive role in ovarian cancer management,70%of patients with newly diagnosis displays at advanced stage due to lacking of effective early diagnosis.The 5-year survival rate has still remained about 30% owing to relapse and resistance after surgery and chemotherapy.Heparanase(Hpa)is known to play an important role in cancer cell invasion by mediating the degradation of extracellular matrix(ECM).In this study,we sought to explore the relationship between Hpa expression and clinic-pathologic pattern in ovarian cancer and also to explore the role of Hpa in invasion and metastatic of ovarian cancer.The relationship between the expression of heparanse mRNA in ovarian carcinomas and it's clinic-pathologyObjective:To explore the human heparanase mRNA expression and its relationship with the clinicopathological characteristics in ovarian carcinomas.Methods:The subjects were 49 patients with ovarian cancer,23 patients with benign ovarian tumor and 22 normal contrlo.The Hpa mRNA expression in different samples were detected by reverse transcription polymerase chain reaction(RT-PCR).Results:(1)The expression of Hpa mRNA was significantly high(P＜0.01)in ovarian cancer than that in benign ovarian tumor and normal ovarian,the expression of Hpa rnRNA was not significantly different(P＞0.05)in benign ovarian tumor and normal ovarian;(2)The expression of Hpa rnRNA was correlated with clinical stages and differentiation degree of cancer cells(P＜0.05).But there was no difference with regard to age,histological type,ascites, lymph node involvement and distant metastasis;(3)Expression of Hpa mRNA was correlative factors with prognosis(P＜0.05);The cumulate survival rate of patient with Hpa positive expression was significantly lower than that of Hpa negative expression.Conclusion:Overexpression of the heparanase mRNA related to ovarian cancer and is relevant to the progressive development of ovarian cancer.The relationship between the expression of heparanse protein in ovarian carcinomas and it's clinic-pathologyObjective:To explore the human heparanase protein expression and its relationship with the clinicopathological characteristics in ovarian carcinomas.Methods:Immunohistochemistry(IHC)was detected the protein of Hpa in 50 cases of ovarian malignant tumor,20 cases of benign ovarian tumor and 20 cases of normal ovarian tissues.The relationship between Hpa expression and clinic-pathologic pattern and prognosis in ovarian cancer was analysed by Kaplan-Meier estimates and Cox regression model.Results:The expression of Hpa was significantly high(P＜0.01)in ovarian cancer than that in benign ovarian tumor and normal ovarian,the expression of Hpa was not significantly different(P＞0.05)in benign ovarian tumor and normal ovarian;The expression of Hpa was correlated with differentiation degree of cancer cells,remainder tumor tissue and lymph node involvement(P＜0.05).But there was no difference with clinical stages,regard to age, histological type,ascites and distant metastasis;The expression of Hpa was higher in patients with lower degree of cancer cell,lymph node involvement and residual disease after surgery was＞2 cm than those in middle-high degree of cancer cell,without lymph node metastasis and residual disease after surgery was＜2 cm(P＜0.05);The cumulate survival rate of patient with Hpa positive expression was no significantly lower than that of Hpa negative expression. In multivariate analyse,gastrocolic omentum involvement remained statistically independent prognostic factors.Conclusion:These results may suggest the over-expression of Hpa may contribute to the development of ovarian tumors.The over-expression of Hpa have significant relevance with the invasion and metastasis of ovarian cancer.Hpa would be as a prognostic indicator in patients with advanced ovarian cancer.Elevated Serum Level of Hpa in Malignant Ovarian Tumor and Its Clinical SignificanceObjective:To study the relationship between the serum levels of Hpa in patients with malignant ovarian tumors.Methods:ELISA(enzyme-linked immunosorbent assay)was detected the serum level of Hpa in 51 ovarian cancers,17 ovarian tumors and 16 normal controls and 10 matched ovarian cancers from pre and post operation.Statistical methods was used to analyze the clinical and pathological characterization by the single-factor analysis of the survival and Cox regression analysis.Results:The serum levels of the Hpa in ovarian cancers was significantly higher than that in the benign tumors and the control group(p＜0.05),significantly higher in pre-operation than that in post operation(p＜0.05),and also in patients with lymph node metastasis was markedly higher than that in patients without lymph node metastasis(p＜0.05).The serum expression of Hpa was associated with clinical tumor stage,tumor grade,ascitic fluid and liver metastasis,but not with histotypes.Correlation analysis showed the relationship between the serum levels of the Hpa and CA125 is direct correlation(r=0.293,P=0.015).Conclusion:The results showed that serum expression levels of Hpa was correlated with carcinogenesis and progress of ovarian cancer and was expected to become as indicators for judging invasion and metastasis.