Research On The Protective Effect Of EGB In Cisplatin-Induced Kidney Damage

Objectives: To establish animal model with cisplatin (cDDP ) induced kidney damage, to investigate the renal toxicity in hares treated with cisplatin ,to investigate the protective effect of Extraxt of ginkgo biloba leaves (EGB) , and to study the mechanism of the protective effect of EGB on cisplatin-induced nephrotoxicity.Method: Twenty-four male New Zealand white hares were randomly divided into four groups: control group (0.9% saline solution), cisplatin (cDDP) group (2.5mg/kg) , low dosage EGB group (cDDP2.5mg/kg + EGB 2mg/kg ) and high dosage EGB group ( cDDP 2.5mg/kg + EGB 4mg/kg ). Their serum samples were measured and their renal tissues were microscopically studied. The changes of Scr , BUN and MDA were measured before injecting drugs and on days 1,3,5 and 7 after injecting drugs. Scr , BUN and MDA were measured by automatic biochemical analysis. One hare selected randomly from every group was killed and its renal tissue structure was observed by light microscopy after 3 days of injecting the drugs.Result: (1) Compared with the control group, the Scr and BUN of the cDDP group was significantly elevated on day 3 , 5 and day 7(P < 0. 05).(2) Compared with the cisplatin group, the Scr and BUN of the high EGB group and the low dosage EGB group were significantly reduced (P < 0. 05).(3)Comparisons of the Scr and BUN in the control group and the low dosage EGB group, the control group and the high dosage EGB group, and between the low dosage EGB group and the high dosage EGB group showed no significant discrepancy (P>0.05).(4)The MDA of all inter group comparisons showed no discrepancy (P> 0.05).(5) Histopathological analysis of the renal tubules in the cDDP group showed scattered areas of edema, degeneration and necrosis, while there was no obvious changes in the glomerulus. In the other 3 groups there was no obvious change in the renal tubules and the glomerulus.Conclusion: (1) The animal models (hares) with weight around 2.0 kg had cDDP (2.5mg/kg) induced kidney damage. (2) EGB can prevent the toxic effect induced by cDDP by lowering the Scr and BUN, thus it can protect the renal structure and function. In our experiment the protective effect of high and low dosages of EGB has no significant difference (P>0.05). (3) Free radical damaging effect was not noticeable by cDDP (2.5mg/kg) induced renal damage.