Enhancement Of Myoblasts Adhesion, Spreading And Proliferation On Biodegradable And Electroactive Triblock Copolymer Of Poly (L-lactide) And Aniline Pentamer With Electric Stimulation And Its Potential Application In Cardiac Tissue Engineering

Biological application of PLA-b-AP-b-PLA (PAP) triblock copolymer of polylactide (PLA) and aniline pentamer (AP) with electroactivity and biodegradability is studied. Biocompatibility of PAP copolymer was investigated by cytotoxicity test, and cell attachment and morphological analysis. The effects of electrical stimulation on myoblasts (H9c2 cell lines) cultured on the thin film surface of the PAP/PLA (1:1) were subsequently investigated by cell proliferation test and ion image analysis. The survival rate of C6 cells in PAP copolymer extract stock solution is higher than those in AP and PLA/AP (1:1). The area fraction and perimeter of H9c2cells on PAP/PLA film surface were the highest, and circularity was the lowest at each time point which proved that the copolymer has favorable biocompatibility. After electrical stimulation (5V, 1Hz, 500ms), the proliferation ratio and intracellular calcium concentration of H9c2 cells on PAP/PLA film surface were improved more significantly than those on PANi, PLA and the glass control at 3 and 6 days culture in vitro. Besides these, something that looks like"pseudopodia"was observed from H9c2 cells on PAP/PLA film surface after stimulation at the 7th day. These results proved that PAP copolymer has well biocompatibility, and properties of electroactive can improve a multitude of cell functions, such as cell attachment, proliferation, migration, and differentiation.