The Expression Of PAH-DNA Adducts In Lung Tissues Of Xuanwei Female Lung Cancer Patients

Objective:To study the expression of PAH-DNA adducts in lung tissues of Xuanwei female lung cancer patients.To explore the relationship between the large number of coal-fired pollution PAHs materials and the high incidence of Xuanwei female lung cancer.Methods:experimental group:20 cases Xuanwei female lung cancer patients.Collect their cancer tissues,adjacent cancer tissues and normal lung tissues each 20 pieces. Control group 1):20 cases Xuanwei male lung cancer patients.Collect their cancer tissues,adjacent cancer tissues and normal lung tissues each 20 pieces.Control group 2):20 cases Non-Xuanwei female lung cancer patients.Collect their cancer tissues, adjacent cancer tissues and normal lung tissues each 20 pieces.Control group 3):10 cases Xuanwei female patients with benign lung lesion.Collect their normal lung tissues 10 pieces.Control group 4):10 cases Non-Xuanwei female patients with benign lung lesion.Collect their normal lung tissues 10 pieces.Use immunofluorescence to detect the expression of PAH-DNA adducts in each group. SPSS 13.0 statistical software was used to analyze the data.Results:The positive expression of PAH-DNA adducts in lung cancer tissues, adjacent cancer tissues and normal lung tissues of Xuanwei female lung cancer patients were 90%,80%and 65%.They were higher than the positive expression of PAH-DNA adducts in Xuanwei male lung cancer patients(35%,30%,30%)and Non-Xuanwei female lung cancer patients(20%,15%,10%)(p＜0.01).The expressions of PAH-DNA adducts in lung tissues of Xuanwei female lung cancer patients and Xuanwei female benign lung lesion patients(positive expression is 70%) were higher than it in Xuanwei male lung cancer patients,Non-Xuanwei female lung cancr patients and Non-Xuanwei female benign lung lesion patients(positive expression is 10%).With the direction changing from cancer tissues,adjacent cancer tissues to normal lung tissues,the expressions of PAH-DNA adducts were decreased but had no statistical difference(p＞0.05).Conclusions:The expressions of PAH-DNA adducts in lung tissues of Xuanwei female lung cancer patients and Xuanwei female benign lung lesion patients were higher than which in Xuanwei male lung cancer patients,Non-Xuanwei female lung caner patients and Non-Xuanwei female benign lung lesion patients.