Experimental Study On Repairing The Rabbits Bone Defects With Bone Marrow Mesenchymal Stem Cells(BMSCs) Combining Nano-hap/collagen(NHAC)

PRATⅠ: The Experimental Study on Inducing Rabbit Bone Marrow Stem Cells into Osteoblast in VitroObjective To study the bone tissue osteogenesis ability of bone marrow stem cells(BMSCs) in vitro in order to offer the ideal seed cells for tissue engineering.Methods Rabbit BMSCs were cultured in vitro induced to osteoblasts,and then the biologic characters of the BMSCs was observed and tested.Results Osteoblast can be induced from BMSCs by conditioned medium, moreover can secret ALP and format the calcific nodules,the image of Factin in osteoblast observed with Congocal Laser Scanning Microscope was red fluorescence.Conclusions BMSCs can be induced successfully to osteoblast in vitro and can be used as ideal seed cells for bone tissue engineering.PRATⅡ: Chondrogenic Induction of Rabbit Bone Marrow Mesenchymal Stem Cells in VitroObjective To study the bone tissue chondrogenic ability of rabbit bone marrow stem cells(BMSCs) in vitro in order to offer the experimental statements for tissue engineering.Methods Rabbit BMSCs were cultured in vitro induced to chondrocytes,and then the biologic characters of the chondrocyte were observed and tested.Results BMSCs became round or ovoid from spindle shape after 7 days exposure to TGF—β1 and vitamin C in conditional culture,then turned into chondrogenic nudoles.The test of Alcian blue staining , safrarine "O" and immunohistochemistry staining for typeⅡcollagen show positive.The test of PCR shows the expression ofⅡcollagen,all revealed the characteristics of chondrogenic differentiation.Conclusions Rabbit BMSCs may be induced into chondrocytes under conditional culture media in vitro.PRATⅢ: The study on the biocompatibility of the nano-hap/collagen with bone marrow stem cellObjective To observe the biocompatibility of BMSCs and NHAC by coculturing them in vitroMethods BMSCs and NHAC were cocultured in vitro for 7 days. After 1,3,7 day,we examined the BMSCs and NHAC by inverted microscope and scanning electron microscpe(SEM).Results The BMSCs can adhere to,proliferate and grow normally and perfectly on the serface and holes of NHAC.Conclusions NHAC has good biocompatibility and can be used as ideal biomaterial in bone tissue engineering.PRATⅣ: Experimental study on repairing the rabbits bone defects with BMSCs combining NHACObjective To explore the impact of NHAC seeded with BMSCs repairing the rabbits bone defectsMethods BMSCs/NHAC composites were implanted the model of 15mm long ulna defects in NEW Zealand white rabbits.At the same time compared with the control group-single NHAC implanting group.At the end of 4,8,12 week after surgery,a series of examinations including X-rays,histomorphology,bone mineral density,bone trabecula area percent,biomechanics and DSA were carried out to analyze the formation of ossicula.Results In the two groups ,the bone critical-sized defects were all repaired successfully.But the time of experimental group was shorter then the control group.At the same examination time point the formation of the vascularzation and ossicula of the experimental group was better than the control group.Conclusions BMSCs/NHAC composites can repair the bone critical-sized defects of the NEW Zealand white rabbits successfully.