Potential Relationship Between Herpes Viruses And Recurrent Aphthous Stomatitis: Analysis With Quantitative Real Time Polymerase Chain Reaction

Twenty recurrent aphthous ulcer(RAU)patients were chosen for this experiment .We collected different samples from them including saliva, exfoliated epithelium cell and blood for real-time fluorescent quantitative PCR(FQ-PCR) test. Through the research we can compare the quantities of virus in different samples and give more explanation of virus causes of RAU.Background and Objective:CD+4/CD+8 is lower in RAU patients. All its recurrent characteristic symptoms that looks like herpes infection and intendance to infect epithelium only imply that the cause of RAU maybe viruses. The objective of researches are all human herpes virus (HHV) recently.The Herpesviridae are a large family of double-stranded DNA viruses that infect a wide range of hosts. Based on biological characteristics, the herpesviruses are classified into three major subfamilies (alpha-, beta-, and gamma herpesviruses), and eight herpesviruses belonging to different subfamilies have been identified as having humans as their primary host.αherpes virus (for example HSV, VZV) can reproduce rapidly and induce to cell disease. They can live in many kinds of hosts. They can infect central nervous system.βherpes virus (for example HCMV,HHV-6,HHV-7) has not many hosts. They can latent in salivary gland, kidney and so on and lead to the latent infection.γherpes virus (for example EBV) have fewer hosts. Most of the aim-cells are B cells. They can latent in them for very long time.The results of researches about RAU involve HHV1-8. For instance, it is reported that many viruses have been found in RAU patients as following HSV-1, VZV, EBV, HCMV, HHV-6, HHV-7, HHV-8, and the difference is notable in statistics, but some researches also found opposite results. Comparing the different experiments, the different ways of operations and collection of samples maybe the keypoint. The aim of this study is to choose real-time fluorescent quantitative PCR to test HHV-6 which has many opposite results and HHV-7 which has fewer results now .Methods:We collected 20 RAU patients in the First Affiliated Hospital of Dalian Medical University, plastic surgery and dental clinic of Dalian medical university. Samples like saliva, epithelium exfoliated cells and blood were collected, at the same time we chose 20 health controls, saliva, exfoliated cells and blood were collected from them. We preserved and took the DNA from them in biochemistry department of Dalian Medical University and samples were sent to TaKaRa company for testing.Results:1)For control group, HHV-6 group and HHV-7 group, positive rates were 10% and 15% and for RAU group were 20% and 35%,which did not exsite statistical differences (P>0.05); 2)HHV-6 and HHV-7 DNA positive rate in saliva were higher than those in PBMCs. 3)In RAU group the HHV-6 virus copies was significantly higher than control group (p<0.05).Conclusions:The results indicated that 1)HHV-6's proliferation occurs in the forepart of the pathological change, its infection correlates to pathogenesis of oral squamous cell in RAU; 2) HHV-6 and HHV-7 has high positive rates in saliva. Saliva is the predominant route of HHV transmission. The method of sampling saliva and detecting HHV-6,7 with PCR is convenient and non-invasive, which provides an efficient screening method.