| Objective: The experiment to observe the percutaneous hepatic artery sustained infusion ofβ-elemene emulsion of rabbit liver transplant VX2 cancer intervention effect ,andβ-elemene emulsion sustained by the artery after reperfusion in rabbits and Pharmacokinetics in the process. Observation ofβ-elemene emulsion sustained in the treatment of liver transplant rabbit VX2 carcinoma of the pathological and Bax, bcl-2 (bcl-2 protein is the regulation of apoptosis, block apoptosis, the extension of survival time, Bax is a cell Apoptosis-regulating genes, the antagonistic role of bcl-2) expression of, thus further explore theβ-elemene anti-tumor mechanism for better application of theoretical basis for the search.Methods: 50 Japanese rabbits successful liver VX2 liver tumor model. Were randomly divided into three groups: (A): 6, 5 hours uniform injection of 50 mg empty milk; treatment group (B): 30, five hours uniform injection of 50 mgβ-elemene, of which 24 remain pharmacokinetics do, 6 and A , C group together to pathology and immunohistochemistry were; C group: six, five hours into uniform 5 - fu 50mg.By femoral artery catheterization, the first catheter placed rabbit liver artery, respectively, according to the different groups in the treatment. Group B after the intervention of 24 were from blood, blood and tumor (made of paste), which measured the concentration. The remaining of group B rabbits and A, C group rabbits in two weeks after the intervention of death, for all tumors, in the light microscope observation of the pathological changes in tumor tissue, immune and bax Detection Group of bcl-2, the evaluation of different options Treatment.Results: 1 specimen: (1) three groups were generally observed differences in tumor weight of larger, The control group has the largest group of heavy, continuous infusion elemene and 5 - Fu group of little difference in weight (2) different levels of necrosis: The treatment group were homogeneous surface of a fish-shaped, the section of the Central District of tumor were different degrees of necrosis, showed Dark yellow rubbish like change, With a small amount of bleeding, swelling of the liver tissues nearby; the capsule tense The control group were more hemorrhage, necrosis lighter, for the performance of small-and survival of tumor necrosis interval organizations exist until the tumor edge, close to no significant change in liver tissue.2 light microscope: the treatment group was solidified tumor necrosis, expressed as a kind of no small granular material, necrotic tissue and complete collapse. Next to that of liver clear water degeneration. Showed significant enlargement of liver cells, plasma osteoporosis, translucent, but still the shape of polygons; tumor and the surrounding areas of vascular thrombosis endometrial thickening of the merger. All treatment groups were to see both inflammatory cell infiltration. One 5 - FU group was moderate necrosis, elemene emulsion infusion group was severe necrosis.3 Immunohistochemistry was: elemene continuous infusion of tumor cells in the Bcl-2 expression was significantly reduced and the expression of Bax greatly increased.Β-elemene group and control group of apoptosis-related gene bcl-2, bax expression main Targeted cancer cells in the cytoplasm and some interstitial tissue, a small number of the membrane at the same time that the cytoplasm and coloring, a stove or flake-like distribution. A small amount of normal liver tissue expression.the expression of bcl-2 positive rate ofβ-elemene group was 10.09±1.23% , 5-FU group was 30.25±4.29%, and blank milk group for 51.58±12.40%, February 2 difference between both Was significant (p <0.05); bax expression ofβ-elemene group and 5 - FU group and empty milk group were 75.29±17.16% and 41.90±9.39% and 18.68±6.44%, February 2 have the difference was significant (p <0.05).β-elemene group and 5 - FU group and empty milk bcl-2/bax ratio of the group were 0.15,0.73,2.85, February 2 have the difference was significant (p <0.05).4 Pharmacokinetics: arterial infusion of blood after the group admitted that the infusion of more than two hours after take after the group, the difference between the two was significant (p <0.05), arterial blood after infusion concentration higher than the group admitted that the infusion After two hours after the group admitted, the two difference was significant (p <0.05), of pulp that is, from an average of pulp group of drug concentrations higher than two hours after the group admitted, but the difference was not significant (p> 0.05) .Conclusions: The percutaneous hepatic artery sustained infusion ofβ-elemene can be effectively treat the rabbit 0f liver transplant VX2 carcinoma. In clinical work, the use ofβ-elemene sustained in the treatment of liver cancer is the choice of method.
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