| Hippocampal is an important tissue during course of learning and memory, and pituitary plays a role in command of the the endocrine system. Aging is the overall performance of the body's function slack up gradually and physiological functions disorder.It is a normal, complicated, and irreversible biological process. With the age of growth, hippocampus and pituitary' s physiological functions gradually diminish, which resulting in brain aging and the endocrine system function decline in signs of aging, such as a drop back in memory, the decline of the body' s immune system and the slow of the metabolism , and finally resulting in the senescence of the whole economy.Previous studies of the hippocampus and pituitary aging mechanism is mainly concentrated in DNA and RNA level or functions of several individual proteins, this kind of research methods and techniques have been unable to meet the post-genome era requirements of research in such a complex phenomenon as senescence.The emergence of the proteomic research aim at all the proteins from cell has already provided new research ideas and technology for senescence. In this study, we choose the aging process in mice hippocampus and pituitary tissue as object. The differential proteomics methods have been used to search for the different expressed proteins during the aging of hippocampus and the pituitary, for the in-depth understanding of their aging mechanism and to provide scientific theory the protein level of verification group D-galactose to the aging brain aging model as a model of the feasibility study and to delay the recession of its function to provide new research methods and clues. This research improved the health status of the elderly and is of great significance to the anti-aging.Firstly,the total protein of the hippocampus from 23-month-old group and 3-month-old mice has been extracted. After the two-dimensional gel electrophoresis, silver staining color, image scanning and PDQuest software analysis, we found the differences in the expression of 45 proteins and with the combination of mass spectrometry analysis and the database search, total of 43 proteins have been identified. During the second part of the experiment , a mice aging model was established in D-galactose induced , from the comparision of the the total protein from aging group and control group and the pituitary the hippocampus, which including the two-dimensional gel electrophoresis, the dye of Coomasie brilliant blue and image analysis, we found 36 different expressed proteins in hippocampus and 46 different expressed proteins in the pituitary .The mass spectrometry analysis was followed and found different expressed proteins in hippocampus and pituitary were 28 and 36 respectively.These results indicate that during the aging process, glucose / energy metabolism dysfunction can cause the lack of functional decline in mitochondrial respiratory chain, ATP generation reduction, ROS generated a lot, and so brain will be more vulnerable to oxidative damage. In addition , VDAC expression is increased in the aging group, the mitochondrial membrane permeability ascend brings the abnormal mitochondrial function, the release of the mitochondrial Cyt C and apoptosis-inducing factor AIF can also cause apoptosis cascade, hippocampus apoptosis caused by nerve Degenerative diseases; CaM also be the important impact of the reduction in learning and memory decline in the aging group. Therefore, the results of this study suggested that oxygen free radicals increase and the Mitochondria-mediated apoptosis are the main reasons for hippocampus and pituitary aging, and to provides a meaningful new clue for the clarity of the molecular mechanism of the hippocampus and pituitary aging. It also proves that the D-galactose aging model is a very good model to the study of hippocampus aging mechanism.
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