Dynamic Expression And Significance Of CD40L And Angâ…¡ Of Perihemotoma Tissue Of Rats With Experimental Intracerebral Hemorrhage

Objective:Through observing the dynamic changes of CD40L and angiotensinⅡ(AngⅡ)positive cell figures of perihemotoma tissue of rat with experimental intracerebral hemorrhage after different treatments during different phases,in order to study their significence in intracerebral hemorrhage and investigate hemotoma local inflammatory response and antiinflammatory effects following intracerebral hemorrhage.Methods:120 healthy SD rats weighing 260-470g,the same male and female numbers,were divided into four groups on average at random:①operative group,②sham operative group,③intervention group,④normal group.The first two groups of rat were injected autologous whole tail blood into basal ganglia regions to simulate intracerebral hemorrhage after anaesthetized,intervention group rats were injected aescinate into abodomen(10mg /kg/d)after operation,The sham operative group rats were injected nromal saline of the equal volume into the same position,normal group rats were raised under the same environment.each group rats were killed respectively at 6h,1d,3d,1w,2w,3w(every time point with 5 rats)after different treatments,perihemotoma tissue and corresponding site tissue of the rats were collected to dye with HE and immunohistochemistry in order to measure expression of CD40L and AngⅡ.statistical software SPSS16.0 and Excel 2003 were applied,Statistical methods mainly include ANOVA, SNK-Q test between group mean,simple linear regression of double variables,statistical significence was set at the value P＜0.05 of probability.Results:1.In shame operative group rat brain tissue HE-dying slices,only a few inflammation cells and little bleeding spots were seen under the optical microscope at acute phase; operative group rat had obvious inflammatory cells infiltrating and hydropic nerve cells and surrouding interstitial tissue,at the 1w,hematoma was absorbed and limited to some extent,inflammatory cells reduced,at the 3w hematoma local pathologic changes were similar to shame operative group;intervention group perihematoma tissue brain hydropic degree and inflammatory cells infiltrating intensity were lower than those of operative group,two groups did not have difference 7d later.2.In normal group,every time point could saw intracerebral hemorrhage corresponding position to express CD40L and AngⅡpositive cells of immunity in neuron and microglia cytoplasm and nucleus under high fold microscopy.,and AngⅡpositive cell were a little more than CD40L positive cell.3.Operative group expression of CD40L and AngⅡreached the maximum in the 1d, later the expressing amount was reduced gradually.4.In sham operative group,every visual field saw a small amount of CD40L and AngⅡimmunizing positive cell that expressed in neuron and microglia cytoplasm and nucleus,but at every time point there were slightly more than normal group in terms of amount,some difference had statistical significance.5.Intervention group postoperative 6h can saw a large amount of AngⅡimmunizing positive cell express,after the 1d of low ebbs,rising again,at the 3d reaching the peak then dropping steadily,expression of CD40L immunizing positive cell peaked in the 1d, dropped steadily hereafter,each the time point intervention group expression of CD40L and AngⅡwere lower than that of operative group before the 1w.6.Within 3w that was observed,at every time point the expression of AngⅡwas higher than that of CD40L,only in operative group there was a moderate correlationship between AngⅡandCD40L immunizing positive cell amount.Conclusions:1.Expression of CD40L and AngⅡof perihemotoma tissue of rat with experimental intracerebral hemorrhage takes on a dynamic change,peaking from the 1d to 3d,dropping gradually later and being identical with brain edema phases. 2.The simple mass effect does not influence on expression of CD40L and AngⅡin intracerebral hemorrhage.3.Expression of CD40L and AngⅡof local perihemotoma tissue does not exist highly degree correlationship,in the inflammatory course of intracerebral hemorrhage,their interaction influences inflammatory response following intracerebral hemorrhage together.4.Aescinate maybe reduce expression of CD40L and AngⅡand resist inflammatory reaction,still requiring to further study.5.Inflammatory responses following intracerebral hemorrhage are complicated, involving a lot of cells,cytokines and molecules,needing to further investigation.