The Feasibility Study On Detecting PG-M3 In Patients With Acute Promyelocytic Leukemia By Flow Cytometry To Diagnosis Differentialy

Objective:To detect the mean fluorescence intensity(MFI) of PG-M3 in acute promyelocytic leukemia(APL)and non-acute promyelocytic leukemia(non-APL).To study evaluation of the differential diagnosis between APL non-APL.Methods:32 patients with AML come from department of hematology in the second hospital of Shanxi medical Universcity.All patients were divided in APL and non-APL.Using FCM to detect the Mean fluorescence intensity(MFI) of PG-M3 in blast cell mass(B) and granular cell mass(G).To calculate rations of MFI between blast cells and granular cells.To statistical analysis the difference of MFI and MFI(B/G) in APL and non-APL.Results:(1).Rations of sMFI(B)(30.04±11.47) in APL group was higher than those (21.73±13.74) in non-APL group,p＞0.05;(2).Rations of cyMFI(270.11±42.94) in APL group was higher than those(243.610±79.483) in non-APL group,P＞0.05;(3).The mean rations of cyMFI(B/G) in APL group was 1.05,higher than those in non-APL group(0.61),P＜0.01(4). There was no significantly reverse relativity in WBC and sMFI(B/G),cyMFI(B/G).Conclusions:(1).sMFI and cyMFI of PG-M3 in patiens with APL was higher than those in patiens with non-APL,but p＞0.05;The sMFI and cyMFI of PG-M3 can not be used as the differential diagnosis critical for APL and non-APL.(2).The mean level(1.05) of cyMFI(B/G) in APL group was higher than those(0.61) in non-APL group,(p＜0.05),The rations of cyMFI(B/G) can be used as the differential diagnosis index in APL and non-APL.It provides important references for diagnosing differentialy in APL(3).There was no significantly reverse relativity in WBC and sMFI(B/G),cyMFI(B/G).(4).The rations of cyMFI(B/G) in individual case was deviated significantly out of the mean level,so it is necessary to combine with morphology,immunology,cytogenetics and molecular biology(MICM) judging differential diagnosis in APL and non-APL. Objective:To analyze characteristic of immunophenotype of acute leukemia;To evaluate the relationship between the expression and CR rate.Methods:Immunopheotypes were examined by multiparameter flow cytometry immunofluoresence methods in 109 cases AL,including AML 73 cases,ALL 23 cases and HAL 12 cases,AUL 1 case.All cases were treated with combined chemotherapy.HA,DA,MA,MAC or IA regime were used to treat AML,ATRA+As₂O₃+low dose DNR regime were used to treat M3; VCAP(VCAD),VDLP(VDLD),VDCP regime were used to treat ALL.VCAAP,MVAP,VDLP regime were used to treat HAL.VADCP regime were used to treat AUL.To analyze characteristic of immunophenotype of acute leukemia and the difference of CR rate between lymphoid antigen positive AML and lymphoid antigen negative AML,the difference of CR rate between myeloid antigen positive ALL and myeloid antigen negative AL(Chisquare test.P＜0.05 was considered as significance).All the statistical variance was carded on SPSS 13.0 in personal computer.Results:â‘ In AML,the incidence of CD13,CD33 and MPO was high,lymphoid antigen CD7,CD19 expressed in AML,cCD79a,cCD3 was negative.CD10,CD7 was negative and the incidence of CD34 and HLA-DR Were low in M3.In ALL,the incidence of lymphoid antigen cCD79a,CD19,CD10 was high,myeloid antigen CD33,CD13 express in ALL.HAL 11.0%of AL coexpress B-lymphoid antigen and myeloid antigen.â‘¡.In AML,CR rate in CD34,HLA-DR,CD19 positive cases was lower than negative cases(p＜0.05).CR rate in CD33 positive cases was higher than negative cases(p＜0.05).CR rate in CD14,CD13,CD7 positive cases was similar to negative cases(p＞0.05).â‘¢In ALL,CR rate in myeloid antigen positive ALL was similar to myeloid antigen negative ALL(p＞0.05).â‘£CR cases in 12 HAL was 41.6%,CR rate was lower thanthose of AML(P＜0.05) and ALL(P＜0.05).Conclusion:â‘ In AML,the incidence of CD13,CD33 and MPO was high;CD10,CD7 was negative and the incidence of CD34 and HLA-DR were low in M3;In ALL,the incidence of lymphoid antigen cCD79a,CD19,CD10 was high;HAL coexpressed B-lymphoid antigen and myeloid antigen mainly.â‘¡Ly+AML and CD34+,HLA-DR+ case may predict a poor clinical outcome.CD7+ was may not the risk factor of clinical outcome.Myeloid antigen and lymphoid antigen were positive in HAL,the curative effect of HAL was poor.