Expression Of Neural Development Gene In The Differentiation Of Adult Neural Stem Cells Into Neurons In Vitro And In Vivo

Objective: To detect the changes and effects of Hes1 and Mash1 gene in the process of bone marrow stromal cells (BMSCs) serial passage and differentiating into neurons. Methods: BMSCs were isolated and cultured with complete bone marrow blood method. Then BMSCs of passage 3(abbreviated to P3) were induced into neurons with DMEM/F12, B27, bFGF and RA. The morphologic features and growth status of BMSCs and differentiated BMSCs were observed by invert microscope. Semi-quantitative reverse transcription polymerase (RT-PCR) was used to measure expressions of Hes1 and Mash1 mRNA in the BMSCs of P3, P5, P7, P9 and P3 BMSCs after induction for 1 and 3 d. Results: The morphology of BMSCs grew well and uniformly after P3 in culture. The morphology of neuron-like cells derived from P3 BMSCs was observed after induction. The expression of Hes1 mRNA was observed to have decreased in a significant and progressive manner and Mash1 mRNA levels significantly increased in P5 BMSCs and then deceased in P7, P9 BMSCs as serial passage. Hes1 mRNA had a temporal decrease at 1 d and then a significant increase at 3 d for induced P3 BMSCs, while Mash1 mRNA had a progressive increase for P3 BMSCs after induction. Conclusion: The Hes1 and Mash1 gene may play an important role in the process of bone marrow stromal cells serial passage and differentiating into neurons. Objective: To study the changes in the expressions of Wnt signaling gene in spinal cord at different time following spinal cord complete transsection injury in rats. Methods: Thirty healthy Wistar rats were randomly divided into 2 groups: sham group (only laminectomy, n=5) and spinal cord injury (SCI) group (n=25). The T11 spinal cord segment of adult rats was transected completely in SCI group. The locomotor functional recovery of hindlimbs was observed by grading the BBB scale. By using semi-quantitative RT-PCR method, the expression of Wnt signaling gene in injured tissue of spinal cord was detected. Results: The hind limbs of rats were completely paraplysis in SCI group and their BBB scales were significantly lower than that in sham group. In SCI group, the expression of Wnt3a gene had remarkable increase both in rostral and caudal to the lesion at postoperative day 1, 3, 7; in caudal side, it was higher than rostral side at the same time and had second increase at 21 d after operation. The expression of Ngn1 was not detected. Hes1 mRNA was significantly decreased at 1 d after operation, an increase at 3 d and then was gradually deceased. Conclusion: Wnt signaling may play an important role in neural regeneration after SCI.