Protein Expression Of MIB1 And TopoⅡα, And Their Relation To Biological Behaver In DLBCLs

Objective To investigate the expression of MIB1 and TopoⅡα, and their relation to the clinical grades and biological behavior in diffused large-B cell lymphomas (DLBCLs).Method 66 cases of DLBCL were selected from the Pathological Deparment of Shanxi Tumor Hospital from 1996 to 2001. An ABC method was used to detecte the expression of CD3,CD20,CD79α,Pax5, and a double immunogolden technique was used to detect the expression of MIB1 and TopoⅡα. Patients related clinical data including follow-up materials were selected.Results Expression of MIB1 and TopoⅡαwere increased along with the clinical stage of the DLBCL. There was a significant difference between more ealy stage (Ⅰ,Ⅱ)and later stage (Ⅲ,Ⅳ) in DLBCL(P<0. 05). The expression of MIB1 and TopoⅡαwere also significantly related to the survival (r=-0·487, 0·251 respectively, all P<0.05), but had no association with ages, genders, B symptoms, LDH level and performance status? (P>0.05).Conclusions Expression of MIB1 and TopoⅡαmay serve as two independent prognostic predictors in DLBCL.