| OBJECTIVE:1. To develop a pyrethroid intragastric administration death model.2. To establish a qualitative and quantitative analysis for pyrethroids.3. To investigate the postmortem distribution of pyrethroid in poisoning death rabbits.METHODS:1. Animal model: Eighteen rabbits were randomly allocated to two groups, one group were divided into three groups named fenpropathrin, fenvalerate, cypermethrin and given a pyrethroid intragastric administration with 2.32ml,7.5ml,10.5ml in even kilogram (n=15 per group ) ;the other group were given the equate volume of physiological salt solution (n=3 per group ).2. Record of vital signs: By a biological function system, the vital signs such as electro cardiac muscle, blood pressure and respiration from beginning of the administration to the death of rabbits were recorded.3. Collection of samples: A rabbits died from the poisoning was dissected as soon as its vital signs disappeared, and then its specimens-the brain, heart, lung, liver, lien, kidney, bile, urine, heart-blood, peripheral blood and gastric wall, as well as body fluid-were collected and sampled immediately. And as soon as it is possible we detected the content of pyrethroid in all tissue and body fluid collected from two groups.4. Analysis: After acid treatment these obtained samples were extracted by trichlormethane. Analysis was performed with a GC equipped with a NPD and a GC-MS. The analyses identification was based on retention time in the chromatographic system coupled with the ion fragmentation spectrum in the mass spectrometer. The quantitative analysis was on an internal standard method.RESULTS: 1. Symptoms : The hair wet, opisthotonos, urinary and fecal incontinence , spasm , decreased corneal reflex , muscular tension muscular tension decrease and dyspnoea occurred at 2~3 hour after an intragastric administration with pyrethroid .And all rabbits from intragastric administration with pesticide group died after 9.97±3.97 h, 6.37±2.71 h and 7.95±3.15 h, but there were nothing occureed in control group.2. The method of gas chromatogram: The study has established the methods of gas chromatogram in detecting diazepam detection. The linear range is 2-400μg/g orμg/ml, the minimum detectable concentration is 0.125μg/g orμg/ml, and the average of recovery rate exceeds 95%.3. The distribution of threee pyrethroids in rabbits: the average content of pyrethroids from high to low: the fenvalerate group : gastric wall, liver, lien, brain, lung, bile, heart, kidney, peripheral blood , heart-blood; the fenpropathrin group: gastric wall, kidney, liver, brain, lien, bile, lung, heart, peripheral blood, heart-blood; the cypermethrin group: gastric wall, liver, kidney, heart, lung, urine, spleen, brain, peripheral blood, heart-blood and bile respectively.CONCLUSION: The study has established one rabbit model of the intragastric administration with pesticide accident death. Three intragastric administration groups died about 9.97±3.97h,6.37±2.71h and 7.95±3.15h after given pesticide respectively . So the established model was available for the forensic identification study on the intragastric administration with pyrethroid accident.The distributions of there pyrethroid pesticides after intragastric administration were similar each every except a few differences. The concentrations of pyrethroid detected were after gastric wall ,liver, lien, brain, lung, bile; gastric wall,kidney, liver, brain, heart, lien; gastric wall ,liver, kidney, heart, lung, urine respectively. The distribution of pyrethroid in intragastric administration rabbits can be applied to the forensic identification study on the intragastric administration with pyrethroid accident. 2. Besides the normal specimen's heart-blood and peripheral blood we still collect other tissues such as liver, kidney, bile, heart, lien and brain in order to be more full-scale equalization and quantitative analysis .Then we get to conclusion combining with symptom and physical sign.
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