The Correlation Between Adiponectin, T-cadherin And STZ-induced Diabetic Nephropathy

Objective: To explore the role of Adiponectin(ADPN) and T-cadherin(T–cad) in the development of diabetic nephropathy(DN) in diabetes mellitus rats. And to provide a new method to diagnose early and therapy DN more effectively.Methods: 64 Wister rats were divided randomly into two groups. 32 rats were in normal control groups and the others were in diabetes mellitus groups. The subjects in diabetes mellitus groups were injected intraperitoneally with dose STZ 50mg/kg while the ones in normal control group only done with the similar dose of citric acid buffer. At 1,4,8 and 12 weeks, weight,fast Blood glucose and 24-hour urinary albumin excretion were observed, serum ADPN,fast blood C-tide were measured, the pathological changes of kidney were examined by light microscope and electron microscope. Renal expression of T–cad was determined by immunohistoehemistry. Meanwhile,the changes of glomerular volume,GBM and immunohistoehemistry were measured by analyzer morphologically. We compare these data with analysis of variance, and analyze the correlation of ADPN and T–cad.Results:1. There was a difference in the increase of body weight between diabetic group and normal control group (P<0.01). The body weight of normal control group increased significantly but diabetic group not. There was a significantly difference in the level of fast blood glucose between two groups (P<0.01). The fast blood glucose of diabetic group sustained at high level and the C peptide maintained at low level after 1week while the control group was normal.2. Compared with the control group, diabetic rats expressed more urinary albumin excretion,12week later, it decreased but was still higher than the control group; path histological study revealed that there was more glomerular matrix and thicker GBM in diabetic rats(P<0.01),the volumes of glomerule of diabetes mellitus groups were higher than the control(P<0.01).3. The blood level of ADPN in diabetic group was higher than that in normal control group after 4weeks (4weeks P<0.05, 8weeks, 12weeks P<0.01), and increased gradually during the whole experiment.4. Compared with normal control group, the expression of T-cad in kidney tissue was strongly in diabetic group, and increased gradually. There was a significantly difference of IDP between two groups after 4weeks (P<0.01). There was a significant positive correlation between ADPN and T-cad in diabetic group(r=0.695,P<0.01).Conclusion:1.The model of STZ-induced diabetic rats was succeed. The pathological changes in myocardial cell were consistent with diabetic renal lesions.2. The blood level of ADPN in diabetic group was more than that in normal control group, and increased gradually with the development of renal lesions. This is similar to the reports of document.3. The expression of T-cad in diabetic group was higher than that in normal control group. There was a significant positive correlation between ADPN and T-cad in diabetic group. With the increase of ADPN level, the expression of T-cad was strongly in kidney tissue.4. From these results, we could suggest that ADPN bind T-cad to protect against apoptosis of kidney cell and neointima hyperplasy. They could protect kidney cell from damage in type1diabetes.