| Objective: To investigate effect of high dose methylprednisolone(MP)on lecithincholesterolacyltransferase mRNA expression after spinal cord injury. Methods: Thirty SD rats were divided equally into operation but none injury group, Allen's weight drop injury group and injury with high dose MP group. Twenty four hours after the operation, the spinal lesion and neighbor areas were moved for examination of lecithin-cholesterolacyltransferase mRNA expression by Retrograde Polymerase Chain Reaction technique (RT-PCR). Results: The lecithin-cholesterolacyltransferase mRNA expression were 0.6743±0.0043, 0.0589±0.0281 (p<0.01), 0.7036±0.2225 (p<0.01) in three group. It increased significantly after spinal cord injury and treatment with MP. Conclusions: MP can greatly increase the expression of lecithin-cholesterolacyltransferase and protect the neighbor areas after spinal cord injury.PART TWO Effect Of High Dose Methylprednisolone On Glycyl Proline Dipeptidyl Aminopeptidase mRNA Expression After Spinal Cord InjuryObjective: To investigate effect of high dose methylprednisolone (MP) on Glycyl Proline Dipeptidyl Aminopeptidase mRNA expression after spinal cord injury mRNA expression after spinal cord injury. Methods: Thirty SD rats were divided equally into operation but none injury group, Allen's weight drop injury group and injury with high dose MP group. Twenty four hours after the operation, the spinal lesion and neighbor areas were moved for examination of Glycyl Proline Dipeptidyl Aminopeptidase mRNA expression by Retrograde Polymerase Chain Reaction technique (RT-PCR). Results: The Glycyl Proline Dipeptidyl Aminopeptidase mRNA expression were 1.5809±0.1554, 0.6605±0.3366 (p<0.01), 1.8502±0.1314 (p<0.01) in three group. It increased significantly after spinal cord injury and treatment with MP. Conclusions: MP can greatly increase the expression of Glycyl Proline Dipeptidyl Aminopeptidase and protect the neighbor areas after spinal cord injury.
|
PDF Full Text Request