Protective Effect Of Peroxisome Proliferator-Activated Receptor γ Activator 15-Deoxyprostaglandin J₂ In Rat Hepatic Ischemia-Reperfusion Injury And Its Mechanism

Objective To investigate the protective effect of peroxisome proliferators-activated receptorγ(PPARγ)activator 15-deoxyprostaglandin J2(15d-PGJ2)in rat hepatic ischemia-reperfusion injury and its mechanism.Methods The model of 70%warm ischemia-reperfusion injury was established in SD rats,rats were randomly divided into 4 groups:Sham operation group,ischemia-reperfusion group,15d-PGJ2 group and 15d-PGJ2+GW9662 group.After reperfusion,AST and ALT levels in serum were determined,the liver tissue was removed for measurement of activity of NF-κB and myeloperoxidase(MPO)and expressions of TNF-αand ICAM-1.Results Compared with sham operation group,the serum levels of ALT and AST and the activity of MPO and NF-κB and expressions of TNF-αand ICAM-1 in ischemia-reperfusion group and 15d-PGJ2 group and 15d-PGJ2+GW9662 group was greatly improved(P＜0.05).Compared with ischemia-reperfusion group,the serum levels of ALT and AST and the activity of MPO and NF-κB and expressions of TNF-αand ICAM-1 in 15d-PGJ2 group significantly decreased(P＜0.05).Compared with 15d-PGJ2 group,the serum levels of ALT and AST and the activity of MPO and NF-κB and expressions of TNF-αand ICAM-1 in 15d-PGJ2+GW9662 group was obviously increased(P＜0.05).Conclusions PPARγactivator 15d-PGJ2 could protect against ischemia-reperfusion injury in rats,with its possible mechanism of inhibiting NF-λB activation and down-regulating TNF-αand ICAM-1 expression in a PPARγdependent fashion.