Effects Of Celery On Mechanism Of Reproductive System In Male Mice

Objective To explore the effects of celery on reproductive system in male mice and its possible mechanism, providing the basis for further development and use of celery.Methods 133 adult male mice of Kunming strain in SPF degree were randomly divided into 4 groups according to the body weight including normal saline group, the low-dose group (Its concentration was about 2.03 g/ml.) ,the medium-dose group (Its concentration was about 4.06 g/ml.) and high-dose group (Its concentration was about 8.12 g/ml.).Mice of each group were divided into 7days, 14days and 28days group. All mice were given corresponding handle with intragastric administration at a certain time every day, experimental group with 0.3ml of celery juice of different concentration and control group with 0.3ml of normal saline. All mice were killed at the end of the experiment.(1) Malondialdehyde (MDA), total antioxide capacity (T-AOC) , superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in testicular homogenate were measured using spectrophotometric assay. (2) Microstructure of testicular was observed under the electron microscope. Testosterone (T), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) of the serum were measured by radioimmunoassay. (3)All kinds of ploidy cells of spermatogenic cells and its cycles were detected by flow cytometry (FCM) (4) Bcl-2 and Bax protein in spermatogenic cells were detected by immunohistochemical method.Result (1) T-AOC of the medium dose group and high dose group were significantly higher than NS group (P<0.005 )in 7 days and 14 days time-groups,but 28 days it had not statistic significance between every dose group in 28 days group.SOD,GSH-Px, MDA and T-AOC had not statistic significance between every dose group in three time-groups.(2) With increasing of dose, the ultra-microstructure damage of testicular cells became more and more serious. Chondriosome dilated and granulations decreased in leydig cells, chondriosome dilated and endocytoplasmic reticulum dilated in seitoli cells and lots of vacuolizations in spermatids were major appearance. The damage of 7days group was more serious than 14days and 28 days. We could observe the necrosis of androgonial cells, primary spermatocytes, spermatids and supporting cells. T, LH and FSH of each group had no statistical significance. (3) Any change of germ-cell cycle in testicle was not found by FCM. (4) Average optical and average gray scale of Bax in every dose group had statistic significance between control groups (P<0.05) in 7d. Average optical and average gray scale of Bcl-2 in every dose group had statistic significance between control groups( P<0.05 )in 28days and the low-dose group had statistic significance (P<0.05 )between the medium-dose group and high-dose group.Conclusion (1) Celery had some effect on the microstructure of testicular,but the effect on the degree of hormone had no statistic significance. (2) Celery could increase the activity of T-AOC to avoid the injury of testicular. (3) The effect of testicular spermatogenic cells was not detected in FCM. (4) Pro-apoptotic role of celery was not observed. Bcl-2 has an increasing role of inhibiting apoptosis of spermatogenic cells.