The Clinical Experimental Research Of Targeting EBV-LMP1 Deoxyribozyme Enhancing The Sensitivity Of NPC Radiotherapy

Objective:Nasopharyngeal carcinoma(NPC)is one of the common maligant tumors in China.Radiotherapy is the main method for its clinical therapy. It is reported that the five-year survival rate of intermediate-advanced stage is less than 50%.Resistance to the therapy,recurrence and metastasis are the main reasons duing to the fail of treatment.Now,to search a new method enhancing radiosensitivity become a hot spot.This study is to use the targeting EBV-LMP1 deoxyribozymes in clinical NPC patients who be diagnosed positive expression of LMP1,observe the effects and side effects of DNAzymes,and investigate targeting EBV-LMP1 DNAzymes may as potential radiosensitizers for NPC treatment.Methods:Divide 20 patients who match the selected conditions into 2 groups in random and double-blind,one is to be tested(Group A,radiotherapy plus the DNAzymes),one is contrast(Group B,radiotherapy alone).The methods of radiotherapy between groups is same:regular radiotherapy using the irregular lead block.The total doses of nasopharynx is DT70-76Gy,accomplished in 7-7.5weeks.The total doses of positive lymph node in cervical is Dr 66-70 Gy,accomplished in 6-7 weeks.2 hours before radiotherapy,Group A be injected the DNAzymes 0.1ml(120mg/ml)in the tumor local,,every Monday and Thursday,and total 10 times.Group B be injected Sodium Chloride 0.1ml the same time. Other treatments are same between 2 groups.Observe the tumor through the endoscope every week,measure the volume through 4 times MRI images rigorously at the point of before the radiotherapy,Dr 50 Gy,D_T70 Gy and return visit after 3 months,test the CR,PR using the solid tumor WHO objective curative effect standards and observe the side effects during the period.Results:The general data is consistency,which means well homogenicity and balanced between 2 groups.Accoding to the tumor volume(cm~3),we changed it into RTR,which means rate of tumor reduction,and carry out two-tailed t test for the values.The results of RTR~3 is P=0.04,which showed a significant difference between two treatments.The results of CR,PR is P=0.092,which can't showed a significant difference between two groups temporarily,as the reason of little cases.The results of the observation index,which from RTOG's acute radiotherapy injured,can't showed a significant difference between two groups.Conclusions:The result suggest that targeting EBV-LMP1 DNAzyme may represent as potential radiosensitizers for NPC treatment.During the period,this DNAzyme did any acute radiotherapy injured,which means well security.