The Expression And Significance Of Interleukin-11(IL-11) And Interleukin-11 Receptor (IL-11R) In Primary Hepatic Carcinoma

Objectives:Previous investigations have shown that interleukin-11(IL-11)and interleukin-11 receptor(IL-11R)have been correlated with the regulation of tumor progression,cellular growth and differentiation in several malignant tumors.The objectives of this study were to clarify the role of IL-11 and IL-11R in human hepatocelluar carcinoma.Methods:30 PHC tissues,their pericarcinomatous liver tissues and their clinicopathological factors were obtained in the current study.Expression levers of IL-11 and IL-11R proteins in samples of pericarcinomatous liver and tumor tissue were analyzed by Western blot.The IOD of IL-11,IL-11R andβ-actin was quantitatively analysised by Analysis software.The expression level of IL-11 and IL-11R was denoted by the ratio of IOD,which was called value A.Results:(1)The value A of IL-11 in PHC tissue is 0.71±0.19,which sharply higher than that in pericarcinomatous liver tissue(0.34±0.27 P＜0.01),The expression of IL-11 has nothing to do with gender,age,size of tumor,corrhosis and lymph node metastasis.There is significance difference between the expression of IL-11 in gradeⅣ(0.83±0.13)and gradeⅠ+Ⅱ+Ⅲ(0.60±0.18)according to Edmondson's grad(P＜0.05).Also the expression of IL-11 is significantly related to capsule invasion(capsule invasion 0.89±0.10 non-capsule invasion 0.64±0.18 P＜0.05)and tumor's thrombus in portal vein(tumor's thrombus in portal vein 0.83±0.13 No tumor's thrombus in portal vein 0.62±0.19 P＜0.05)(2)The value A.of IL-11R in PHC tissue is 0.56±0.19,which remarkable higher than that in pericacinomatous liver tissue(0.41±0.25 P＜0.01).The expression of IL-11R have no relationship with gender,age,size of tumor,cirrhosis and differentiation grade of tumor.But there is statistical difference between the expression of IL-11R in tumor with lymph node metastasis(0.70±0.09)and tumor without lymph node metastasis(0.50±0.19 P＜0.05).Also the expression of IL-11R is significantly related to capsule invasion(capsule invasion 0.72±0.09 non- capsule invasion 0.48±0.17 P＜0.05)and tumor's thrombus in portal vein(tumor's thrombus in portal vein 0.67±0.15 No tumor's thrombus in portal vein 0.49±0.16 P＜0.05)Conclusion:(1)The expression of IL-11 and IL-11R is upregulated obviously in PHC tissue,which indicate that IL-11/IL-11R may be concerned with the occurrence of PHC.(2)IL-11's expression is markly higher in Edmonson gradingⅣ, capsule invasion,tumor's thrombus in portal vein group than that in Edmonson gradingⅠ+Ⅱ+Ⅲ,non-capsule invasion,No tumor's thrombus in portal vein group.IL-11R's expression is obviously higher in lymph node metastasis,capsule invasion,tumor's thrombus in portal vein group than that in non-metastasis non-capsule invasion,No tumor's thrombus in portal vein group.These findings suggest that IL-11/IL-11R pathway may play important role in the differentiation,invasion and transfer of PHC.