The Role Of FOLR1 On The Process Of Paclitaxol-resistance In Nasopharyngeal Carcinoma Cells

Objectives: To explore the role of FOLR1 playing in the process of paclitaxol-resistance in nasopharyngeal carcinoma cellsMethods: The expression of FOLR1 gene was detected by cDNAmicroarray, RT-PCR and Immunocytochemistry in CNE-1 and paclitaxel-resistance cells (named CNE-1/Taxol). Cell morphology was observed under inverted microscope, and the growth inhibition rate of cells was assayed by MTT experiments after CNE1 and CNE1/Taxol treated by monoclonal antibody of FOLR1. The sensitivity to paclitaxel of cells was assessed by MTT experiments.Results : The results of gene chip revealed that the expression of FOLR1 gene in the paclitaxel-resistance nasopharyngeal carcinoma cells was 50 times higher than in the corresponding sensitive cell line. The higher expression of FOLR1 in CNE1/Taxol was verified by semi-quantative RT-PCR, and its expression level was positive co-related to the degree of drug-resistance. The growth of CNE1/Taxol could be significantly inhibited by high dose of FOLR1 antibody, and the rate of growth inhibition was positive related to the concentration of antibody. The sensitivity to paclitaxel of CNE-1/Taxol was significantly increased by treatment of low dose FOLR1 antibody.Conclusions: The expression of FOLR1 gene was close related to the paclitaxol-resistance in nasopharyngeal carcinoma cells .The specific monoantibody of FOLR1 could significantly inhibite the growth of CNE1/Taxol, and increase the sensitivity to paclitaxol in CNE1/Taxol, and reverse the drug resistance of CNE1/Taxol. It indicated that the FOLR1 gene is one of the important target molecules in the reversion of the paclitaxel-resistance in nasopharyngeal carcinoma cell.