Initial Study Of HBV Genetic Characteristic In Liver Cirrhosis/Hepatocellular Carcinoma

【Objetive】Hepatitis B virus is a major causative agent to lead to chronic hepatitis,liver cirrhosis and liver cancer.The certain mechanism that HBV lead to disease is not distinct,the experiment studies the correlative of HBV genic heterogeneity and liver cirrhosis or HCC because of HBV,and screening a HBV genotyping that apply to large sample screening and clinical application.【Methods】Randomly select 85 end Stage liver disease patients about HBV, all patients prepared to do liver transplantation in Tianjin First Center Hospital from May 2006 to Aug 2007.The number of the posthepatitic cirrhosis was 32,the number of HCC was 53.Liver functional parameter of all patients was detected by automatic biochemistry analyzer,blood clotting functional parameter by automatic blood clotting analyzer;HBV serumal marker was detected in immunologic method;HBV DNA quantitation was detected by HBV real time fluorescent quantitation PCR. Venous 4 ml blood from all patients dissociate serum and collect clinical data. Patients serum HBV fixed quantity with HBV real time fluorescent quantitation PCR. Randomly selected 30 patients from 85 end stage liver disease patients,The number of the posthepatitic cirrhosis was 12,the number of HCC was 18.The HBV S gene was amplified by PCR,the PCR product was cut by enzyme.The results was compared with genome genotyping.The PCR-RFLP genotyping is a perfect method.【Results】Analyze liver function parameter,HBV serumal marker,HBV DNA quantitation about 85 end stage liver disease patients and the discrepancy is compared between two groups.PT,TB was significantly higher in LC group than in HCC group,Fib,GGT were significantly lower in LC group than in HCC group(P＜0.01);"little triple yang"incidence rate and the number of HBV DNA quantitation≥1.0×10~5 copies/ml were higher in HCC group than in LC group(P＜0.05).Mutations are most resided in EnhⅠ,EnhⅡ,BCP,precore,Ⅹgene by HBV DNA genomic sequencing of 30 patients.The aberration rate of C1155T(58.3%),C1173G/T(58.3%) in EnhⅠand A1727G/T(75.0%)in EnhⅡare higher in LC group than in HCC group. In HCC group the aberration rate of T1753G/C(61.1%),A1762T/G1764A(66.7%)in BCP and C1386G(72.2%),A1574T/C(44.4%)inⅩgene are higher than in LC group. PCR-RFLP genotyping is precise,sensitiveness is to 10~4 copies/ml.The number of C genotype in HCC group is more than in LC group.【Conclusions】The number of patients which reflected HBsAg(+),HBeAb(+),HBcAb(+),HBV DNA quantitation≥10~5 copies/ml,C genotype in HCC group was more than in LC group;The number of patients which reflected mutations of C1155T,C1173G/T in EnhⅠ,mutations of A1727G/T in EnhⅡin LC group was more than in HCC group;concerned with liver cirrhosis about HBV;Compared with patients in LC group,mutations of T1753G/C,A1762T/G1764A in BCP,mutations of C1386G,A1574T/C inⅩgene in HCC group was more;PCR-RFLP genotyping is precise,sensitive and apply to large sample screening and clinical application.