| Objectives:To investigate the expression of HDAC1,p300 and p21WAF1and correlations among them in gastric carcinoma and normal mucosa adjacent to carcinoma,we want to explore their roles in gastric carcinoma and supply theoretical basis for diagnosis, treatment and prognosis of gastric carcinoma.Methods:Immunohistochemistry was performed to detect the expression of HDAC1, p300 and p21WAF1in 42 specimens of gastric carcinoma and normal mucosa adjacent to carcinoma.All data were analyzed by SPSS 11.5 software.Results:1.The positive rate of expression of HDAC1,p300 and p21WAF1in gastric carcinoma and normal mucosa adjacent to carcinoma was 59.52%,35.71%;45.24%, 69.04%and 40.48%,64.29%respectively.2.The positive rate of expression of HDAC1 in gastric carcinoma was significantly higher than normal mucosa(P＜0.05),while the positive rate of expression of p300 and p21WAF1in gastric carcinoma were statistically lower than normal mucosa(P＜0.05).3.The positive rate of expression of HDAC1 in poorly-differentiated adenocarcinoma was obviously higher than well-differentiated and moderately differentiated adenocarcinoma(P＜0.05),the positive rate of expression of HDAC1 in gastric carcinoma with lymph node metastasis was higher than that without lymph node metastasis(P＜0.05).4.The positive rate of expression of p300 in gastric carcinoma with T3 and T4 infiltrative depth was significantly lower than gastric carcinoma with T1 and T2 infiltrative depth(P＜0.05),the positive rate of expression of p300 in gastric carcinoma with lymph node metastasis was statistically lower than that without lymph node metastasis(P＜0.05).5.The positive rate of expression of p21WAF1in gastric carcinoma with T3 and T4 infiltrative depth was strikingly lower than gastric carcinoma with T1 and T2 infiltrative depth(P＜0.05),the positive rate of expression of p21WAF1in gastric carcinoma with lymph node metastasis was statistically lower than that without lymph node metastasis(P＜0.05).6.In gastric carcinoma,the expression of HDAC1 was negatively correlated with the expression of p21WAF1(r=-0.407,P＜0.05),the expression of p300 was positively correlated with the expression of p21WAF1(r=0.323,P＜0.05),but there was no correlation between the expression of HDAC1 and p300(P＞0.05).7.In normal mucosa adjacent to carcinoma,the expression of HDAC1 was negatively correlated with the expression of p21WAF1(r=-0.348,P＜0.05),the expression of p300 was positively correlated with the expression of p21WAF1(r=0.361, P＜0.05),but there was no correlation between the expression of HDAC1 and p300(P＞0.05).Conclusions:1.High expression of HDAC1 in gastric carcinoma was observed and it was correlated with the pathologic differentiation degree and lymph node metastasis.Our reasearch suggests that HDAC1 promotes the development,infiltration and lymph node metastasis of gastric carcinoma.2.Low expression of p300 and p21WAF1in gastric carcinoma was observed and it was correlated with infiltrative depth and lymph node metastasis.It suggests that low expression of p300 and p21WAF1could be one of the important causes which affected the infiltration and lymph node metastasis of gastric carcinoma.3.The expression of HDAC1 in gastric carcinoma and normal mucosa adjacent to carcinoma was negatively correlated with the expression of p21WAF1.It shows that HDAC1 may have a inhibitory effect on p21WAF1by histone deacetylation, then accelerate the development of gastric carcinoma.4.The expression of p300 in gastric carcinoma and normal mucosa adjacent to carcinoma was positively correlated with the expression of p21WAF1,so p300 may promote the expression of p21WAF1by histone acetylation,then prevent the development,infiltration and metastasis of gastric carcinoma.5.Because the expression of p21WAF1was regulated by HDAC1 and p300,HDAC1 and p300 can be involved in cell cycle by regulating the expression of p21WAF1in the development of gastric carcinoma.6.HDAC1,p300 and p21WAF1may be the molecule markers of gastric cancer and be used in diagnosis,prognosis and therapy of gastric carcinoma.
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