The Experiment Research On Traditional Chinese Medicine Of Yiqihuoxue, Huayutongluo In Renal Interstitium Fibrotic

Objective:Tubulointerstitial fibrosis (TIF) is a common pathological feature of most end-stage kidney disease. Numbers of clinical pathology observations had shown that tubulointerstitial injury played an more important role than glomerular damage on renal failure. It is the TIF's cause that Disequilibrium of extracellular martix (ECM) composition and degradation lead to a great quantity ECM's accumulation. Clinically,Angiotensin Converting Enzyme Inhibitor(ACEI)and Angiotensin II ReceptorAntagonist(ARB) etal are applied to prevent and cure TIF by adjusting synthesis and degrada -tion of ECM and transformation of myofibroblast(Myo-FB). However the medical science had testified that above-mentioned higher price medicines not only can't protect kidney,but also might lesion it in Scr higher than certain numerus,Therefore, to search the medicine that have many channels, many targets, safe and low cost treatment, there is a world of significance to prevent and cure TIF. In the experiment, The TIF that unilateral ureteral obsurtction(UUO) rat model induce to are treated by advisor's empirical formula- Longjiashenqi soup,and contrast with ARB- Valsartna to observe the change that Longjiashenqi soup effect observeexperimental animal Obstruct kidney's transforming growth factorβ₁(TGF-β₁), collagenIII(ColIII) and histomorphology so as to approach Chinese medicinal formulae Longjiashenqi soup antagonism and mechanism of action in TIF.Methods: Fourty female Sprague-Dawley rats were randomly divided into four groups(n=10), Shma-operation (SHAM), model(UUO), Longjiashenqi soup (UUOL) and Valsartna (UUOV). Each animal was ligated left ureter except SHAM group. Durgs were administered from the day before operation but the saline were used in SHAM and UUO groups by oral. Animals were sacrificed after twenty-one days the left kidneys were harvested for pathological study. Imm -unohistochemisyrt staining were used for expression of TGF-β₁, ColIII,and to apply patho-image analysis software to do semi-quantitative analysis.Results:1 The detection result on Index,SCr,BUN1.1 At the end of experiment, left kidney volume is increasing,the Index on UUO group was higher than that on SHAM group(p<0.01);compared with the UUO group,The Index of UUOV,UUOL groups decreased marked ly (p<0.05, p <0.05). but there was not statistically significant between them(p>0.05).1.2 The SCr of UUO group was higher than that on SHAM group(p<0.05);compared with the UUO group,the SCr of UUOV,UUOL groups decreased markedly(p<0.05, p<0.05). but there was not statistically significant between them(p>0.05).1.3 The BUN of UUO group was higher than that on SHAM group(p<0.05);compared with the UUO group,the BUN of UUOV,UUOL groups decreased markedly(p<0.05, p<0.05). but there was not statistically significant between them(p>0.05).2 The renal tissues were stained with HE and Tianlangxing -hong:There was no change in SHAM group.But in UUO group,interstitial macrophage and monocyte infiltra -tion,renal tubular atrophy,emphraxis or expand were shown. The number of glomeurli reduced markedly,and the area of glomeurli decreased.The renal capsule adhered or expanded. Fibrous tissues proliferated around the renal cpasule.While the lesions in treatment groups were slighter than that in UUO group.3 The expression,distribution and semi-quantitative analysis of TGF-β₁: It was shown a weak expression for TGF-β₁ in SHAM group,while in other groups the expression of TGF-β₁ inereased. They can be seen in kidney proximal tubule, cellula epithelialis endochylema and interstitial cell. The Positive area and density of TGF-β₁ in UUO group was higher than that in SHAM group(p<0.01);compared with the UUO group,the Positive area and density of TGF-β₁in UUOV ,UUOLgroups decreased markedly(p<0.01,p<0.01). It means that Longjiashenqi soup and Valsartan can inhibit the expression of TGF-β₁ in obstructive kindye.The inhibition of UUOV group was stronger than that of UUOL group,but there was not statistically significant between them(p>0.05).4 The expression, distribution and semi-quantitative analysis of ColIII: They can be seen in renal interstitium. It was shown a weak expression for ColIIIin SHAM group,while in other groups the expression of ColIII inereased.The Positive area and density of ColIII in UUO group was higher than that in SHAM grouP(p<0.01);compared with the UUO group,the Positive area and density of ColIII in UUOV, UUOLgroups decreased markedly(p<0.01,p<0.01). It means that Longjiashenqi soup and Valsartan can inhibit the expression of ColIII in obstructive kindye.The inhibition of UUOV group was stronger than that of UUOL group,but there was not statistically significant between them(p>0.05).5 In UUO group,the area of fibrosis in UUO group were more serious than in Sham group; the area of fibrosis in UUOV, UUOL groups were decreased markedly compared with the UUO group (p<0.01,p<0.05).It means that Longjiashenqi soup and Valsartan can inhibit fibrosis in obstructive nephro pathy. Among them , the inhibition of UUOL group was stronger than that of UUOVgroup,but there was not statistically significant between them(p>0.05).Conclusion:1 Longjiashenqi soup can degrade serum creatinine and urea nitrogen, protect renal function.2 Longjiashenqi soup can restrain expression of TGF-β₁, ColIII in nephridial tissue so as to reduce pathological changes synthesis and accumulation of ECM, finally, it can delay TIF's proceeding.3 Longjiashenqi soup's intervention effect to TIF of the UUO rat is similar to Valsartan's.