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Preparation And Aggreation Study Of Nanometer Perfluorocarbon Lipid Ultrasound Contrast Agent

Posted on:2009-11-12Degree:MasterType:Thesis
Country:ChinaCandidate:S ChenFull Text:PDF
GTID:2144360245488464Subject:Medical imaging and nuclear medicine
Abstract/Summary:PDF Full Text Request
PART I A NOVEL PERFLUOROCARBON LIPID MICROPHERE:PREPARATION AND AGGREGATION STUDYObjective: To develop a novel nanometer ultrasonic contrast agent—perfluorocarbon lipid microspheres and investigate their basic properties and the peculiarity of the enhancing ultrasound imaging when aggregating .Methods: Rotary evaporation and high pressure homogen were used to preparate perfluorocarbon lipid microspheres, and the appearance and distribution of the particles investigated by microscope and electron microscope, the concentration calculated by blood cell counting chamber and the size and electric potential detected. And a biotinylated perfluorocarbon lipid particle was prepared for ultrasound imaging before and after adding stepavidin. The average gray scale value of the captured ultrasound images were analyzed by the DFY-II型software.Results: These nanoparticles are uniform and stabilized, and the size is about 170 nm. As to the ultrasound experiment, there was no visuable ultrasound signals scattered back from the particles before adding strepavidin, while obviously ultrasound echo signals were observed after adding strepavidin. The statistical significance of the the ultrasound images after/before adding strepavidin could be analyzed by DFY-II型software.Conclusions: A steady perfluorocarbon lipid microspheres was prepared and the biotinylated microspheres could aggregate together in the existence of strepavidin and enhance the ultrasound image. The aggregated microspheres might be taken as a kind of ultrasound contrast agent, which lay the foundation for the later research. PART II TARGETING STUDY IN VITRO OF HUMAN HEPATOCELLULAR CARCINOMA TARGETED PERFLUOROCARBON LIPID MICROPHEREObjective : To develop perfluorocarbon lipid microspheres and investigate their basic properties, and lead by hepatoma monocolonal antibody HAb18, use avidin-biotin interaction to target human hepatocellular carcinoma cell in vitro.Methods: Rotary evaporation and high pressure homogen were used to preparate perfluorocarbon lipid microspheres, and the appearance and distribution of the microspheres investigated by microscope and electron microscope, the concentration calculated by blood cell counting chamber and the size and electric potential detected. Then prepared the biotinylated monoclonal antibody HAb18. The number of biotin molecules coupled to each antibody were determined and the immunological activity of biotinylated HAb18 was tested by ELISA. After preparing the biotinylated perfluorocarbon lipid microspheres labelled in NBD, avidin-biotin interaction was use to target human hepatocellular carcinoma cell in vitro.Results:Green fluorescence was observed at the edges of the biotinylated perfluorocarbon lipid microspheres labelled in NBD. ELISA indicated the immunological activity of biotinylated HAb18 was similar to that of free HAb18. 7721 cells were targeted to surround by the biotinylated microspheres labelled in NBD based on biotin-avidin system.Conclusions:The biotinylated perfluorocarbon lipid microspheres and biotinylated HAb18 were prepared successfully, and these biotinylated microspheres can target to bind to 7721 cells based on biotin-avidin system.
Keywords/Search Tags:Nanometer, Perfluorocarbon, Ultrasound contrast agent, Perfluorocarbon, Monocolonal, Biotin, Avidin
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