| Objective: To explore the effect of ceftriaxone sodium administered by amniocentesis in treatment of intrauterine infection with bacteria in late pregnant rats,and to evaluate the dose-response relationship and safety of ceftriaxone sodium administered by amniocentesis to treat the rat model.Methods: (1) 36 SD pregnant rats for 15 days were randomly divided into four groups (9 in each), E coli suspension inoculated into one horn of uterus of each rat. On the 16-days, each gestational sac in inoculation side of rats in intrauterine administration (IUA) group and control (IUC) group was given drug or appropriate volume of isotonic Na chloride (NS) by amniocentesis, and each rat in maternal intravenous injection (IVA) group and control (IVC) group was given ceftriaxone sodium or appropriate volume of isotonic NS by intravenous. From the 17th day, 3 rats of each group were sacrificed randomly and respectively everyday. Still birth rate will be calculated. Amniotic fluid and placenta of the top 3 sacs in inoculation side of each rat will be detected, E coli negative rate and mild chorioamnionitis rate were computed. One of the top 3 sacs in inoculation side of each rat randomly selected, the expression of IL-6 and MMP-9 in placenta analyzed semiquantitively by immunohistochemical method, and the apoptoic degree of trophoblasts detected semiquantitively by TUNEL. (2) 24 SD pregnant rats for 15 days inoculated in the same way were randomly divided into four groups (9 in each). On the 16-day, each 4 rats in IUA and IVA groups were given appropriate low, medium and high dose of ceftriaxone sodium respectively, and were sacrificed the next day to obtain the indexes above.Results: (1) The still birth rates in total and the 3rd day after administration were lower in IUA compared with IUC, but the reversed result of E coli negative rates. The E coli negative rates and mild chorioamnionitis rates of IUA in total and the 1st day after administration were higher than those of IVA. Everyday respectively after administration, the level of IL-6 and MMP-9 in each treatment group was lower than it in its control group (P<0.05), The apoptosis level of trophoblasts was lower in IUA group than in IVA group on the 1st and 3rd after administration (P<0.05). (2) There were no significance (P>0.05) in incidence of fetal death between intrauterine administration and maternal intravenous route of each dosage groups, also among each dose group of both routes. The E coli negative rate and the mild chorioamnionitis rate of both routes rising along with the rise of dosage, but no significant between medium and high grades in IUA group(P>0.05). The mild chorioamnionitis rate was higher in intrauterine administration route of each dosage grade, with a lower expression of IL-6 and MMP-9 in placenta, and a lower level of trophoblasts apoptosis as well (P<0.05), following a decending level of the later 3 indexes gradually related to the rised dosage in each route.Conclusions: Ceftriaxone sodium administrating with a single medium dosage by amniocentesis is effective and economical for treating intrauterine infection with E coli in late pregnant SD rats without a rising of still birth rate, which can inhibit E coli growth, release chorioamnionitis more quickly, and inhibit the expression of IL-6 and MMP-9 in placenta, suppress and postpone the inflammation induced apoptosis of trophoblasts as well, so as to reduce the level of inflammation and the risk of premature rupture of the membranes. In addition, its administration by amniocentesis in therapeutical dose range is acceptable safe according to our study. |
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