Determination Of Nine β₂-agonists Residues In Urines By Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

As the fast development of economy, food derived from animal is becoming more and more important in our life, but drug residues derived from food in every kind of animal have become a hot topic of public health. In this paper, an analytical method based on ultra performance liquid chromatography-tandem mass spectrometry with positive mode has been developed for the simultaneous determination ofβ₂-agonists in urines. Samples were deconjugated withβ-glucuronidase/sulfatase enzyme in acetate buffer, deproteinized by perchloric acid and the pH was adjusted to 4.0. The concentration and purification was performed using Oasis HLB, Oasis MCX. The separation was performed on a Waters ACQUITY UPLC^TM BEH C18 column (100 mm×2.1 mm, 1.7μm) with gradient elution using methanol and 0.1 % formic acid at a flow rate of 0.3 mL/min. The limits of detection (LOD) of the method ranged from 0.002 to 0.02μg/L and the limits of quantification (LOQ) ranged from 0.007⁰.06μg/L. Average recoveries at the spiking levels of 0.1, 0.5 and 2μg/L ranged from 75.3 % to 116.0 %, with relative standard deviations of within-run between 3.4 % and 27.4 % and relative standard deviations of between-run between 5.2 % and 24.5 %.