| Objective Zhang et al performed SSH(suppression subtractive hybridization)on control HUVECs(human umbilical vein endothelial cells)versus HUVECs exposed to tumor-conditioned media.They found that a novel cDNA(GenBank Accession No. AF358829)is differentially expressed in endothelial cells on Northern analysis,and named it endothelial-derived gene-1(EG-1).There is still no research about correlation between EG-1 and thyroid cancer all over the world.In this research,we use RT-PCR to investigate the expression of EG-1 in thyroid cancer,further more,we analyze the relationship between gene expression and clinical prognosis of the patients.In order to find a good predictor for evaluating the prognosis of thyroid cancer.Methods Archived specimens of thyroid cancer patients who were treated in Shandong provincial hospital between Jan 2000 and Aug 2002 and were followed up routinely were obtained.All the patients were confirmed diagnosis of thyroid cancer by post operation pathology.52 cases which were randomly selected to be brought into the study were examined EG-1 expression by RT-PCR,15 cases of benign tumors were the control.The deadline of follow-up was May,2007,the median follow-up time was 70(8～87)months.Data of patients who were died of other causes, or out of follow-up,or survived till the end of follow-up were calculated censored. SPSS 10.0 software was used to statistical analysis.Kaplan—Meier curve was used to analyze monofactors survival,and cox proportion ratio regression model was used to analyze poly factors survival.Statistical significance was defined by p＜0.05.Results(1)EG-1 expression in 38 cases of malignant and 4 cases of benign thyroid tissues at the mRNA levels were evaluated by RT-PCR.The EG-1 expression was much higher in malignant tissue than in the corresponding benign tumor tissue at the mRNA levels.(2)Monovariate survival analysis showed that overall survival of EG-1 positive patients were significantly shorter than EG-1 negative patients.Meanwhile, the overall survival between different groups of age,lymphonode metastatic,tumor grade,and pathologic type were significantly different.(3)In a multivariate Cox model in which overall survival was evaluated,EG-1 provided independent significant predictive power,as well as age,lymphonode metastatic,tumor grade,and pathologic type.(4)To evaluate the value of three scoring systems in predicting the progonosis of thyroid cancer,in this test,Monovariate survival analysis showed they were all useful.Conclusions EG-1 is preferentially expressed in thyroid cancer and may be used as an important prognostic marker of it. Objective At present,a large number of breast cancer related oncogenes have been found and some of them have been demonstrated to be useful to the clinical investigation.However,the oncogene playing critical role in the progress of breast cancer has not been found so far.Liu et al performed SSH(suppression subtractive hybridization)on control HUVECs(human umbilical vein endothelial cells)versus HUVECs exposed to tumor-conditioned media.They found that a novel cDNA is differentially expressed in endothelial cells on Northern analysis,and named it endothelial-derived gene-1(EG-1).Their data suggested that EG-1 was associated with a stimulated state in endothelial and epithelial cells,and may have a role in tumor angiogenesis.There is still no research about correlation between EG-1 and breast cancer clinical characteristics,although the laboratory study have proven many results.Herein,we will do some preliminary work for future research.This study is designed to demonstrate expression of EG-1 in breast cancer and to correlate the EG-1 expression level with prognosis of breast cancer.Methods Archived specimens of breast cancer patients who were treated in Shandong provincial hospital between Oct,1995 and Aug,1999 and were followed up routinely were obtained.All the patients received radical breast cancer surgery and axillary lymph node dissection,and were all confirmed diagnosis of invasive breast cancer by post operation pathology.72 cases which were randomly selected to be brought into the study were examined EG-1 expression by RT-PCR,18 cases of benign tumors were the control.Immunohistochemistry were used to examine the expression of p53,Her-2 and VEGF protein.The deadline of follow-up was 1 st Oct,2006,the median follow-up time was 89(32～123)months.During the period of follow-up,38(52.7%)patients occurred relapse(25 cases of distant relapse,13 case of local relapse),among which,26(36.1%)patients died from breast cancer.4(5%) patients died of cardiovascular diseases and accidents.42(58.3%)patients survived till the end of follow-up.Data of patients who were died of other causes,or out of follow-up,or survived till the end of follow-up were calculated censored.SPSS 10.0 software was used to statistical analysis.The analysis of Chi-square test was performed for comparison of EG-1 expression between breast cancer specimens and benign tumor specimens.Kaplan—Meier curve was used to analyze monofactors survival,and cox proportion ratio regression model was used to analyze poly factors survival.Statistical significance was defined by p＜0.05.Results 1.EG-1 expression in 72 cases of malignant and 18 cases benign breast tissues at the mRNA levels were evaluated by RT-PCR.The EG-1 expression was much higher in malignant tissue than in the corresponding benign breast tissue at the mRNA levels(χ~2=17.604,p＜0.01).2.Monovariate survival analysis showed that overall survival and disease free survival of EG-1 positive patients were significantly shorter than EG-1 negative patients(p＜0.05).3.In a multivariate Cox model in which overall survival and disease free survival were evaluated,EG-1 provided independent significant predictive power,as well as positive lymph node numbers and Her-2 protein.However,protein VEGF only predicted disease free survival.Conclusions The EG-1 expression is up-regulated in breast cancer.It may be an important tumor biomarker and therapeutic target.
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