Effect Of Titanium Alloy's Regular Surface Microtopography On The Biological Behavior Of Fibroblast In Vitro

Titanium alloys have been widely used in orthopedic and adjacent subjects because of their favourable biocompatibility, mechanical funcation and corrosion resistance. At present,there are two prodominant problems exist in commercial titanium alloys: one of them is the difficulty of effective integrate between the interface of biomatierals and bone or soft tissues;the other is the stress shield created by the high elastic modulus of implants.Reshaping the suface to improve the biocompatibility of implants is a reliable method in order to solve the interface problem, sudies have showed that the titanium alloy implants treated with surface reshaping would be good to integrate with bone and soft tissues, among these methods surface morphology reshaping was widely concerned for it's excellent contact guidance effect and surface stability. Certainly we should improve our manufactural technical to upgrade the modulus of biomaterials. There is no report of histiocyte compatibility reseaches about the titanium alloys with regular microtopographied surface.Our study plans to observe the Effect of titanium alloy's different surface microtopography on the biological behavior of rabbit fibroblast,and investigate the early integrate mechanism of these alloy's regular surface microtopography with soft tissues.1. The study about the culture,puration and growth character of rabbit lacertus fibroblastObjective Finding a convenient and reliable cultivation to obtain pure fibroblasts, and study their character of growth and proliferation in vitro. Methods Cultured by methods of explant and passaged after purition, rabbit lacertus fibroblast's growth condition and cell morphous were observed,growth curve was drew and the cell's doubling time was calculated. Results By methods of explant, stable and pure fibroblasts with high reproductive activity were obtained. Conclusion Explant is a convenient and feasible method for cell culture,by this mean we can get enough seeds of fibroblast to afford the correlate research.2. Effect of titanium alloy's ragular surface microtopography on the biological behavior of rabbits'lacertus fibroblast in vitroObjective To study the effect of different surface microtopography of titanium alloy on biological behavior and soft tissue integration of the rabbits'lacertus fibroblast. Methods The lacertus fibroblasts of Achilles tendon of rabbits were cultured and inoculated on the surface of smooth control group and sand blast,microgrooves,thread Ti-flakes. The cell's growth ratio and attaching status of different surface were examined by MTT test and scanning electron microscope(SEM).Results The rabbits'lacertus fibroblasts proliferated regularly on the smooth,sand blast,microgrooves and thread surface, their intercomparison were statistical significance(P<0.05),this indicated that different surface microtopography had different effect on cell's proliferation; By the observation of SEM ,the cells had not obvious directivity on smooth surface,they attached irregular on sand blast surface but oriented regularly along the texture of the other two group of samples. Cells were almost shuttle-like on the four kind of surface except for a part of irregular cells on sand blast surface, and there were plentiful cell-cell junctions. Under high power lens ,we could find cells with limpid microvillus in experiment samples. Conclusion The microtopography surface of titanium alloy was suitable for proliferation and adherence of fibroblasts of rabbit, cells proliferated the fastest on the sand blast surface but their attachment were disordered,however, the regular microgrooved surface was more suitable for the requirement of the soft tissues'physiological function because of the cell's fast proliferation and regular attachment.