| The Endocrine disrupting chemicals (EDCs)are exogenous material which candisturb hormone synthesis, secretion, transport and effect. They are lipophilicify andhard to degradation. It can damage human's reproduction. The Di-(2-ethylhexyl)phthalate (DEHP)is a kind of plasticizer that has hormone activity has made severepollution all over the world. DEHP can disturb male's reproductive ability specificallyand it can influence prostate grown, development, differentiation and cause prostatediseases. So we designed this experiment to research the relationship between DEHPand prostate.Thirty-three SD rats were divided into four groups randomly : normal controlgroup , low dose DEHP group [50 mg/(kg·d)] , middle dose DEHP group[(200mg/(kg·d)], and high dose DEHP group [500 mg/(kg·d)]. Different dosages of DEHPwere administered to SD rats with injection of abdominal cavity from the fifth weeksafter birth to thirteen weeks . After the rats were killed , the prostate was dissected andweighted , the prostate volume was measured and the prostate index was calculated.Prostatic tissues were stained by hemoloxylin, eosin and immunohistochemistry, thenmorphological changes of prostate tissues were observed by light microscope. In comparison with normal group , prostatic volumes and weight as well as theprostate index were significantly increased ( P < 0. 05) with glands expanded andinterstitial tissues increased, and the degree of prostatic hyperplasia show a positivecorrelation with the dose of DEHP. Exposure to DEHP on male rat during the period ofadolescent age may induce prostate hyperplasia which manifests enlargement ofglandular lumen accompanied by hyperplasia of interstitial tissues.The prostate is biggest sex gland organ and it is controlled by sex hormone. TheDEHP is a kind of EDCs, so it disturb the normal sex hormone's function. So we thinkthe DEHP can lead prostate hyperplasia, but the mechanisms we need to investigatefurther. Retroperitoneal bleeding is very common in clinic. Because of the space ofretroperitoneal is very huge and deep, it can contain many blood, so clinicalmanifestation is very complicated. It can induce very severe consequences andcomplications, even death. But the diagnostic methods of retroperitoneal bleedinginclude CT, ultrasound, MRI etc. But all of those methods can't monitor the patients'real time bleeding, they can't be used at bedside. It's very high risks for the patients whocan't move. So we need a equipment that can continue monitoring the bleeding. Now,the electrical impendence tomography (EIT) is a new tool to monitor bleeding.The EIT base on the cellular membrane is a kind of insulation membrane that havevery low electrical leakage character. Different tissues, even different condition of sametissues have different electrical impedances. So we can use this character to make thetomography to monitor the bleeding. This equipment is non-invasive, it can monitor theelectrical impedances of tissues that we can know the physiological functions andpathological conditions of tissues. This equipment is suitable, no radiation, non-invasiveand can get the bleeding message real time, all of those characters are very difficult for most medical equipments.We use the EIT technology to monitor retroperitoneal bleeding and we use twodifferent models, the retroperitoneal inject blood model and retroperitoneal bleedingmodel. We want to reveal the relationship between retroperitoneal bleeding and EIT, andwe hope to find a new method to diagnose retroperitoneal bleeding. The first model, weinsert a vessel into retroperitoneal and inject blood to simulate retroperitoneal bleedingand use EIT monitoring the pigs. When we finish monitoring, all pigs are inspected byCT and anatomy. The second model, pigs retroperitoneal bleeding model is establishedby the puncturatio of kidney from outside with fine needles guided by ultrasound, andpigs are heparinized at same time. All those pigs are inspected by CT and anatomy too.The 5 pigs of retroperitoneal inject blood model are all survival when the monitor isover. All pigs'anesthesia are steady. The CT tomography reveal that the rightretroperitoneal of pigs have the vessel and hematoma, and the hematoma is localized.The organs in abdominal cavity are integrity and no blood or fluid in there. The anatomyresults find all pigs'post peritonaeum is integrity, no blood in abdominal cavity, and onehuge hematoma is in retroperitoneal cavity. The vessel between the kidney and greaterpsoas muscle, the kidney, greater psoas muscle and great vessels are not injured. Thegray scale of EIT images are uniformity before we inject blood and the gray scale of EITimages change enormously when the more and more blood are injected intoretroperitoneal cavity. We can see the area that the electrical impendence decreasegradually, and the images are steady when we change the injector.The 5 pigs of retroperitoneal bleeding model are all survival when the kidney hasbeen punctured three hours. The CT and anatomy all find that every pigs'postperitonaeum is integrity, no blood in abdominal cavity, and the volume of hematoma inretroperitoneal cavity is from 100ml~180ml, bleeding rate is 100%. All the other organs and vessels are not injured. When EIT start monitoring, the gray scale of EIT images areuniformity and decrease enormously when the monitoring time prolong. The area isconsistent with the CT and anatomy.The retroperitoneal inject blood models have same site to puncture, and the modelsparallel the real retroperitoneal bleeding. We inject blood with determinate time andspeed, so we can get precise relationships between images and blood volume. We cancontrol the speed, blood volume, so we can repeat the experiment easily. Theretroperitoneal bleeding models cause kidney bleeding is very similar real retroperitonealbleeding. The EIT equipment is simple, suitable and reliable. The consequences of twomodels reveal the models are successful, they can fit the need to study the retroperitonealbleeding.The EIT can monitor the retroperitoneal bleeding sensitively and can monitor thebleeding area, the images are clear and easy to read. The contrast increases when theblood volume increase or bleeding time prolong. Because of its simple, reliable, noninvasiveand no radiation, the EIT refer to long time monitoring at bedside.
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